Analysis of the alternative pathways for the beta-oxidation of unsaturated fatty acids using transgenic plants synthesizing polyhydroxyalkanoates in peroxisomes.

Détails

ID Serval
serval:BIB_B24BBD7034CA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Analysis of the alternative pathways for the beta-oxidation of unsaturated fatty acids using transgenic plants synthesizing polyhydroxyalkanoates in peroxisomes.
Périodique
Plant Physiology
Auteur(s)
Allenbach L., Poirier Y.
ISSN
0032-0889 (Print)
ISSN-L
0032-0889
Statut éditorial
Publié
Date de publication
2000
Volume
124
Numéro
3
Pages
1159-1168
Langue
anglais
Résumé
Degradation of fatty acids having cis-double bonds on even-numbered carbons requires the presence of auxiliary enzymes in addition to the enzymes of the core beta-oxidation cycle. Two alternative pathways have been described to degrade these fatty acids. One pathway involves the participation of the enzymes 2, 4-dienoyl-coenzyme A (CoA) reductase and Delta(3)-Delta(2)-enoyl-CoA isomerase, whereas the second involves the epimerization of R-3-hydroxyacyl-CoA via a 3-hydroxyacyl-CoA epimerase or the action of two stereo-specific enoyl-CoA hydratases. Although degradation of these fatty acids in bacteria and mammalian peroxisomes was shown to involve mainly the reductase-isomerase pathway, previous analysis of the relative activity of the enoyl-CoA hydratase II (also called R-3-hydroxyacyl-CoA hydro-lyase) and 2,4-dienoyl-CoA reductase in plants indicated that degradation occurred mainly through the epimerase pathway. We have examined the implication of both pathways in transgenic Arabidopsis expressing the polyhydroxyalkanoate synthase from Pseudomonas aeruginosa in peroxisomes and producing polyhydroxyalkanoate from the 3-hydroxyacyl-CoA intermediates of the beta-oxidation cycle. Analysis of the polyhydroxyalkanoate synthesized in plants grown in media containing cis-10-heptadecenoic or cis-10-pentadecenoic acids revealed a significant contribution of both the reductase-isomerase and epimerase pathways to the degradation of these fatty acids.
Mots-clé
Acyltransferases/genetics, Acyltransferases/metabolism, Arabidopsis/enzymology, Arabidopsis/metabolism, Fatty Acid Desaturases/metabolism, Fatty Acids/chemistry, Fatty Acids/metabolism, Fatty Acids, Unsaturated/chemistry, Fatty Acids, Unsaturated/metabolism, Oxidation-Reduction, Oxidoreductases Acting on CH-CH Group Donors, Peroxisomes/enzymology, Peroxisomes/metabolism, Plants, Genetically Modified, Polyesters/chemistry, Polyesters/metabolism, Pseudomonas aeruginosa/enzymology, Pseudomonas putida/metabolism, Racemases and Epimerases/metabolism, Stereoisomerism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 19:42
Dernière modification de la notice
20/08/2019 15:21
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