Diagnostic Value of Histological Analysis of Punch Biopsies in Suspected Cutaneous Buruli Ulcer: A Study on 32 Cases of Confirmed Buruli Ulcer in Cameroon.

Détails

Ressource 1Télécharger: BIB_B2273C4282B5.pdf (799.63 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_B2273C4282B5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Diagnostic Value of Histological Analysis of Punch Biopsies in Suspected Cutaneous Buruli Ulcer: A Study on 32 Cases of Confirmed Buruli Ulcer in Cameroon.
Périodique
Dermatopathology
Auteur(s)
Ibrahim Y.L., Masouyé I., Tschanz E., Atangana P., Etard J.F., Serafini M., Mueller Y.K., Toutous Trellu L.
ISSN
2296-3529 (Print)
ISSN-L
2296-3529
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
6
Numéro
2
Pages
28-36
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Buruli ulcer (BU) is a cutaneous infectious disease caused by Mycobacterium ulcerans. In this prospective study, we aim to clarify the main histopathological features of cutaneous BU based on 4-mm skin punch biopsies and to evaluate the diagnostic value of this method.
Between 2011 and 2013, a prospective study was conducted in Cameroon. Dry swabs from ulcerative lesions and fine-needle aspirates of nonulcerative lesions were examined for Ziehl-Neelsen (ZN) staining, followed by PCR targeting IS2404 and culture. Two 4-mm punch biopsies were performed in the center and in the periphery of each lesion.
The 364 patients included in the study had 422 lesions (381 were ulcerative and 357 lesions were biopsied). Among the 99 ulcerated lesions with a final diagnosis of BU, histological features for BU were fulfilled in 32 lesions. 32/32 showed subcutaneous necrosis with a neutrophilic inflammatory infiltrate. 26/32 presented alcohol-resistant bacilli confirmed by ZN stain on histology.
Punch biopsies help in establishing the correct diagnosis of BU and also in the differential diagnosis of chronic ulcers. The main histological feature for BU is diffuse coagulative necrosis of subcutaneous tissue, with acid-fast bacilli detected by ZN stain.
Mots-clé
Buruli ulcer, Histology, Prospective study
Pubmed
Open Access
Oui
Création de la notice
30/06/2019 15:51
Dernière modification de la notice
20/08/2019 15:20
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