Immunohistochemical characterization of human retinoblastomas in situ with multiple markers

Détails

ID Serval
serval:BIB_B18391917F40
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immunohistochemical characterization of human retinoblastomas in situ with multiple markers
Périodique
American Journal of Ophthalmology
Auteur(s)
Perentes  E., Herbort  C. P., Rubinstein  L. J., Herman  M. M., Uffer  S., Donoso  L. A., Collins  V. P.
ISSN
0002-9394 (Print)
Statut éditorial
Publié
Date de publication
05/1987
Volume
103
Numéro
5
Pages
647-58
Notes
Journal Article --- Old month value: May 15
Résumé
We studied paraffin-embedded specimens from 18 surgically enucleated eyes with retinoblastoma by peroxidase-antiperoxidase immunohistochemistry with antibodies against glial fibrillary acidic protein, S-100 protein, Leu 7 epitopes, neuron-specific enolase, the 200-kilodalton subunit of the neurofilament triplet polypeptide, and retinal S-antigen. We found that (1) glial fibrillary acidic protein, S-100 protein, and Leu 7 epitopes were detected only in well-differentiated glial cells that were interpreted as reactive and not neoplastic, (2) undifferentiated neoplastic cells expressed both neuron-specific enolase and retinal S-antigen immunoreactivity, and (3) differentiated cells forming Flexner-Wintersteiner rosettes were found to express neuron-specific enolase, retinal S-antigen, and, occasionally, neurofilament protein. These results support the view that retinoblastomas are composed of neuron-committed cells and favor the origin of these tumors from photoreceptor progenitor cells. We did not find any morphologic or immunohistochemical evidence of glial differentiation from tumor cells that would support the concept that retinoblastoma arises from a primitive neuroectodermal cell capable of divergent differentiation along neuronal and glial lines.
Mots-clé
Antibodies, Monoclonal/immunology Eye Neoplasms/analysis/*immunology Glial Fibrillary Acidic Protein/analysis Humans Immunoenzyme Techniques Phosphopyruvate Hydratase/analysis Retinoblastoma/analysis/*immunology S100 Proteins/analysis
Pubmed
Web of science
Création de la notice
28/01/2008 13:02
Dernière modification de la notice
20/08/2019 15:20
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