Simian virus 40 revertant enhancers exhibit restricted host ranges for enhancer function.
Détails
ID Serval
serval:BIB_B12A7CA3EABF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Simian virus 40 revertant enhancers exhibit restricted host ranges for enhancer function.
Périodique
Journal of Virology
ISSN
0022-538X[print], 0022-538X[linking]
Statut éditorial
Publié
Date de publication
09/1988
Volume
62
Numéro
9
Pages
3364-3370
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Résumé
We have assayed the cell-specific activity of a matched set of four enhancers found in viral revertants derived from simian virus 40 (SV40) enhancer mutants. These enhancers all contain 71-base-pair duplications that span identical regions or, in one case, the same region shifted by 2 nucleotides. The four enhancers differ, however, in that each one either carries a different wild-type pair of the genetically defined SV40 enhancer A, B, or C elements, with the other two elements mutated, or carries all three elements mutated. The three enhancers carrying two copies of a wild-type element effectively enhance transcription in CV-1 and HeLa cells, but only the enhancer containing a duplicated wild-type C element exhibits activity in NIH 3T3 cells. These results show that the ability of the A, B, and C elements to compensate for one another is cell specific and that selection for enhancer function in one cell type can generate enhancers with different cell-specific activities. These results are consistent with the hypothesis that tandem duplication of multiple distinct enhancer elements, as in wild-type strains of SV40 (e.g., the 72-base-pair repeat), has the property of expanding the host range of an enhancer.
Mots-clé
Animals, Base Sequence, Cell Line, Enhancer Elements, Genetic, Hela Cells, Humans, Mutation, Plasmids, Simian virus 40/genetics, Transcription, Genetic, Transfection
Pubmed
Web of science
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 16:20