Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria

Détails

ID Serval
serval:BIB_B11D9749CBDD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Critical role of lipopolysaccharide-binding protein and CD14 in immune responses against gram-negative bacteria
Périodique
Journal of Immunology
Auteur⸱e⸱s
Le Roy  D., Di Padova  F., Adachi  Y., Glauser  M. P., Calandra  T., Heumann  D.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
09/2001
Volume
167
Numéro
5
Pages
2759-65
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep 1
Résumé
LPS-binding protein (LBP) and CD14 potentiate cell activation by LPS, contributing to lethal endotoxemia. We analyzed the contribution of LBP/CD14 in models of bacterial infection. Mice pretreated with mAbs neutralizing CD14 or LBP showed a delay in TNF-alpha production and died of overwhelming infection within 24 h, after a challenge with 250 CFU of virulent Klebsiella pneumoniae. Blockade of TNF-alpha also increased lethality, whereas pretreatment with TNF-alpha protected mice, even in the presence of LBP and CD14 blockade. Anti-LBP or anti-CD14 mAbs did not improve or decrease lethality with a higher inoculum (10(5) K. pneumoniae) and did not affect outcome following injections of low or high inocula of Escherichia coli O111. These results point to the essential role of LBP/CD14 in innate immunity against virulent bacteria.
Mots-clé
*Acute-Phase Proteins Animals Antibodies, Monoclonal Antigens, CD14/*metabolism Bacteremia/etiology/immunology Carrier Proteins/antagonists & inhibitors/*immunology/*metabolism Endotoxemia/immunology Escherichia coli/immunology/pathogenicity Female Gram-Negative Bacteria/*immunology/pathogenicity Gram-Negative Bacterial Infections/etiology/immunology Klebsiella pneumoniae/immunology/pathogenicity Lipopolysaccharides/*metabolism/toxicity *Membrane Glycoproteins Mice Neutralization Tests Neutrophils/immunology Sepsis/etiology/immunology Tumor Necrosis Factor-alpha/biosynthesis/pharmacology Virulence
Pubmed
Web of science
Création de la notice
25/01/2008 14:28
Dernière modification de la notice
20/08/2019 16:20
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