Erythroferrone as a sensitive biomarker to detect stimulation of erythropoiesis.

Détails

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Etat: Public
Version: Author's accepted manuscript
Licence: Non spécifiée
ID Serval
serval:BIB_B10331793EDC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Erythroferrone as a sensitive biomarker to detect stimulation of erythropoiesis.
Périodique
Drug testing and analysis
Auteur(s)
Ramirez Cuevas K., Schobinger C., Gottardo E., Voss SC, Kuuranne T., Tissot JD, Favrat B., Townsend N., Leuenberger N.
ISSN
1942-7611 (Electronic)
ISSN-L
1942-7603
Statut éditorial
Publié
Date de publication
02/2020
Peer-reviewed
Oui
Volume
12
Numéro
2
Pages
261-267
Langue
anglais
Notes
Publication types: Journal Article ; Randomized Controlled Trial
Publication Status: ppublish
Résumé
Erythroferrone (ERFE) is a glycoprotein hormone secreted by erythroblasts in response to erythropoietin stimulation. ERFE suppresses the hepatic synthesis of the master iron-regulatory hormone, hepcidin. The impact of erythropoiesis stimulation on ERFE secretion in humans is poorly understood. This paucity of information is due in part to the lack of available means for ERFE quantification in serum samples. The present study tested a new sensitive sandwich immunoassay for human ERFE. This assay was used to demonstrate that injection of various erythropoiesis stimulating agents (ESAs) increased the blood ERFE levels in healthy volunteers. After exogenous stimulation of erythropoiesis, ERFE increased up to 8-fold with a detection window of 13 days. The impact of one unit of blood withdrawal on erythropoiesis stimulation of ERFE was also tested. ERFE significantly increased after blood withdrawal in subjects injected with both iron and saline solution, suggesting that iron supplementation did not mask the ERFE increase after blood withdrawal. The effects of exercise-induced muscle damage on ERFE was assessed by comparing ERFE levels with creatine kinase levels in samples from subjects with heavy exercise loads, and determined that this was not a confounder. The ERFE assay is a sensitive means to investigate the connection between iron metabolism and erythropoiesis in humans, and to detect ESA abuse in the antidoping field.
Mots-clé
Adult, Biomarkers/blood, Erythropoiesis/drug effects, Erythropoietin/administration & dosage, Erythropoietin/pharmacology, Exercise, Hematinics/administration & dosage, Hematinics/pharmacology, Humans, Injections, Iron/administration & dosage, Iron/pharmacology, Male, Peptide Hormones/blood, Peptides/administration & dosage, Peptides/pharmacology, Substance Abuse Detection/methods, Young Adult, blood doping, erythroferrone, immunoassay
Pubmed
Web of science
Création de la notice
30/10/2019 15:57
Dernière modification de la notice
04/02/2021 6:25
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