Photoprotective potential of lycopene, beta-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts

Détails

ID Serval
serval:BIB_B0CAF0B97B8B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Photoprotective potential of lycopene, beta-carotene, vitamin E, vitamin C and carnosic acid in UVA-irradiated human skin fibroblasts
Périodique
Free Radical Biology and Medicine
Auteur(s)
Offord  E. A., Gautier  J. C., Avanti  O., Scaletta  C., Runge  F., Kramer  K., Applegate  L. A.
ISSN
0891-5849 (Print)
Statut éditorial
Publié
Date de publication
2002
Volume
32
Numéro
12
Pages
1293-1303
Notes
DA - 20020611
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Antioxidants)
RN - 0 (Biological Markers)
RN - 0 (Diterpenes)
RN - 0 (Diterpenes, Abietane)
RN - 0 (Membrane Proteins)
RN - 0 (Plant Extracts)
RN - 0 (Radiation-Protective Agents)
RN - 1406-18-4 (Vitamin E)
RN - 36-88-4 (Carotenoids)
RN - 3650-09-7 (salvin)
RN - 50-81-7 (Ascorbic Acid)
RN - 502-65-8 (lycopene)
RN - 7235-40-7 (beta Carotene)
RN - EC 1.14.99.3 (HMOX1 protein, human)
RN - EC 1.14.99.3 (Heme Oxygenase (Decyclizing))
RN - EC 1.14.99.3 (Heme Oxygenase-1)
RN - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB - IM
Résumé
The photoprotective potential of the dietary antioxidants vitamin C, vitamin E, lycopene, beta-carotene, and the rosemary polyphenol, carnosic acid, was tested in human dermal fibroblasts exposed to ultraviolet-A (UVA) light. The carotenoids were prepared in special nanoparticle formulations together with vitamin C and/or vitamin E. Nanoparticle formulations, in contrast to dimethylsulphoxide, stablized lycopene in the cell culture medium and allowed efficient cellular uptake. The presence of vitamin E in the formulation further increased the stability and cellular uptake of lycopene. UVA irradiation of the human skin fibroblasts led to a 10-15-fold rise in metalloproteinase 1 (MMP-1) mRNA. This rise was suppressed in the presence of low microM concentrations of vitamin E, vitamin C, or carnosic acid but not with beta-carotene or lycopene. Indeed, in the presence of 0.5-1.0 microM beta-carotene or lycopene, the UVA-induced MMP-1 mRNA was further increased by 1.5-2-fold. This increase was totally suppressed when vitamin E was included in the nanoparticle formulation. Heme-oxygenase 1 (HO-1) mRNA expression was strongly induced by UVA irradiation but none of the antioxidants inhibited this effect at the concentrations used in this study. Indeed, beta-carotene or lycopene (0.5-1.0 microM) led to a further 1.5-fold rise in the UVA-induced HO-1 mRNA levels. In conclusion, vitamin C, vitamin E, and carnosic acid showed photoprotective potential. Lycopene and beta-carotene did not protect on their own but in the presence of vitamin E, their stability in culture was improved and the rise in MMP-1 mRNA expression was suppressed, suggesting a requirement for antioxidant protection of the carotenoids against formation of oxidative derivatives that can influence the cellular and molecular responses
Mots-clé
Adult/analysis/Antioxidants/Ascorbic Acid/beta Carotene/Biological Markers/Blotting,Northern/Carotenoids/Cells,Cultured/Chromatography,High Pressure Liquid/Cytoprotection/Diterpenes/Diterpenes,Abietane/DNA Damage/drug effects/Fibroblasts/Heme Oxygenase (Decyclizing)/Heme Oxygenase-1/Humans/Light/Male/Matrix Metalloproteinase 1/Membrane Proteins/metabolism/pharmacology/Plant Extracts/Proteins/radiation effects/Radiation-Protective Agents/Skin/Switzerland/Ultraviolet Rays/Vitamin E
Pubmed
Web of science
Création de la notice
18/02/2008 17:33
Dernière modification de la notice
20/08/2019 15:19
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