Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior.
Détails
ID Serval
serval:BIB_B09A00A6B4B0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparative impact on islet isolation and transplant outcome of the preservation solutions Institut Georges Lopez-1, University of Wisconsin, and Celsior.
Périodique
Transplantation
ISSN
1534-6080 (Electronic)
ISSN-L
0041-1337
Statut éditorial
Publié
Date de publication
15/04/2012
Peer-reviewed
Oui
Volume
93
Numéro
7
Pages
703-708
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
Institut Georges Lopez-1 (IGL-1) is a preservation solution similar to University of Wisconsin (UW) with reversed Na/K contents. In this study, we assessed the impact of IGL-1, UW, and Celsior (CS) solutions on islet isolation and transplant outcome.
We retrospectively analyzed 376 islet isolations from pancreases flushed and transported with IGL-1 (n=95), UW (n=204), or CS (n=77). We determined isolation outcome and β-cell function in vitro. Transplanted patients were divided into three groups depending on preservation solution of pancreas, and islet graft function was assessed by decrease in daily insulin needs, C-peptide/glucose ratios, β-scores, and transplant estimated function at 1- and 6-month follow-up.
IGL-1, UW, and CS groups were similar according to donor age, body mass index, and pancreas weight. There was no difference in islet yields between the three groups. Success rates, transplant rates, β-cell secretory function, and viability were similar for all three groups. We observed no difference in decreased insulin needs, C-peptide glucose ratios, β-scores, and transplant estimated function at 1- and 6-month follow-up between IGL-1, UW, and CS groups.
Our study shows that IGL-1 is equivalent to UW or CS solutions for pancreas perfusion and cold storage before islet isolation and transplantation.
We retrospectively analyzed 376 islet isolations from pancreases flushed and transported with IGL-1 (n=95), UW (n=204), or CS (n=77). We determined isolation outcome and β-cell function in vitro. Transplanted patients were divided into three groups depending on preservation solution of pancreas, and islet graft function was assessed by decrease in daily insulin needs, C-peptide/glucose ratios, β-scores, and transplant estimated function at 1- and 6-month follow-up.
IGL-1, UW, and CS groups were similar according to donor age, body mass index, and pancreas weight. There was no difference in islet yields between the three groups. Success rates, transplant rates, β-cell secretory function, and viability were similar for all three groups. We observed no difference in decreased insulin needs, C-peptide glucose ratios, β-scores, and transplant estimated function at 1- and 6-month follow-up between IGL-1, UW, and CS groups.
Our study shows that IGL-1 is equivalent to UW or CS solutions for pancreas perfusion and cold storage before islet isolation and transplantation.
Mots-clé
Adenosine/therapeutic use, Adult, Allopurinol/therapeutic use, Analysis of Variance, Biomarkers/blood, Blood Glucose/metabolism, C-Peptide/blood, Chi-Square Distribution, Diabetes Mellitus/blood, Diabetes Mellitus/drug therapy, Diabetes Mellitus/surgery, Disaccharides/therapeutic use, Electrolytes/therapeutic use, Female, Glutamates/therapeutic use, Glutathione/therapeutic use, Glycated Hemoglobin/metabolism, Histidine/therapeutic use, Humans, Hypoglycemic Agents/therapeutic use, Insulin/therapeutic use, Islets of Langerhans/drug effects, Islets of Langerhans/metabolism, Islets of Langerhans Transplantation, Male, Mannitol/therapeutic use, Middle Aged, Organ Preservation/methods, Organ Preservation Solutions/therapeutic use, Raffinose/therapeutic use, Retrospective Studies, Time Factors, Tissue Culture Techniques, Tissue and Organ Harvesting/methods, Treatment Outcome
Pubmed
Web of science
Open Access
Oui
Création de la notice
14/06/2021 9:59
Dernière modification de la notice
24/05/2024 12:38