Evaluation of uptake and transport of ultrasmall superparamagnetic iron oxide nanoparticles by human brain-derived endothelial cells.

Détails

ID Serval
serval:BIB_AF87980CE4C4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Evaluation of uptake and transport of ultrasmall superparamagnetic iron oxide nanoparticles by human brain-derived endothelial cells.
Périodique
Nanomedicine (london, England)
Auteur⸱e⸱s
Kenzaoui B.H., Bernasconi C.C., Hofmann H., Juillerat-Jeanneret L.
ISSN
1748-6963 (Electronic)
ISSN-L
1743-5889
Statut éditorial
Publié
Date de publication
01/2012
Peer-reviewed
Oui
Volume
7
Numéro
1
Pages
39-53
Langue
anglais
Résumé
Aim: Ultrasmall superparamagnetic iron oxide nanoparticles (USPIO-NPs) are under development for imaging and drug delivery; however, their interaction with human blood-brain barrier models is not known. Materials & Methods: The uptake, reactive oxygen species production and transport of USPIO-NPs across human brain-derived endothelial cells as models of the blood-brain tumor barrier were evaluated for either uncoated, oleic acid-coated or polyvinylamine-coated USPIO-NPs. Results: Reactive oxygen species production was observed for oleic acid-coated and polyvinylamine-coated USPIO-NPs. The uptake and intracellular localization of the iron oxide core of the USPIO-NPs was confirmed by transmission electron microscopy. However, while the uptake of these USPIO-NPs by cells was observed, they were neither released by nor transported across these cells even in the presence of an external dynamic magnetic field. Conclusion: USPIO-NP-loaded filopodia were observed to invade the polyester membrane, suggesting that they can be transported by migrating angiogenic brain-derived endothelial cells.
Pubmed
Web of science
Création de la notice
05/01/2012 10:55
Dernière modification de la notice
20/08/2019 15:19
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