Herpesvirus saimiri transforms human T-cell clones to stable growth without inducing resistance to apoptosis

Détails

ID Serval
serval:BIB_AF85911DCEFA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Herpesvirus saimiri transforms human T-cell clones to stable growth without inducing resistance to apoptosis
Périodique
Journal of Virology
Auteur⸱e⸱s
Kraft  M. S., Henning  G., Fickenscher  H., Lengenfelder  D., Tschopp  J., Fleckenstein  B., Meinl  E.
ISSN
0022-538X (Print)
Statut éditorial
Publié
Date de publication
04/1998
Volume
72
Numéro
4
Pages
3138-45
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
Herpesvirus saimiri (HVS) transforms human T cells to stable growth in vitro. Since HVS codes for two different antiapoptotic proteins, growth transformation by HVS might be expected to confer resistance to apoptosis. We found that the expression of both viral antiapoptotic genes was restricted to cultures with viral replication and absent in growth-transformed human T cells. A comparative examination of HVS-transformed T-cell clones and their native parental clones revealed that the expression of Bcl-2, Bcl-X(L), Bax, and members of the tumor necrosis factor receptor (TNF-R) superfamily with a death domain, namely, TNF-RI, CD95, and TRAMP, were not modulated by HVS. Expression of CD30 was induced in HVS-transformed T cells, and these cells also expressed the CD30 ligand. Uninfected and transformed T cells were sensitive to CD95 ligation but resistant to apoptosis mediated by TRAIL or soluble TNF-alpha. CD95 ligand was constitutively expressed on transformed but not uninfected parental T cells. Both cell types showed similar sensitivity to cell death induction or inhibition of T-cell activation mediated by irradiation, oxygen radicals, dexamethasone, cyclosporine, and prostaglandin E2. Altogether, this study strongly suggests that growth transformation by HVS is based not on resistance to apoptosis but, rather, on utilization of normal cellular activation pathways.
Mots-clé
Animals *Apoptosis Base Sequence Cell Cycle Cell Division Cell Line, Transformed *Cell Transformation, Viral DNA, Viral Genes, Viral Haplorhini Herpesvirus 2, Saimiriine/genetics/*physiology Humans Molecular Sequence Data Proteins/metabolism T-Lymphocytes/cytology/*virology
Pubmed
Web of science
Création de la notice
24/01/2008 15:19
Dernière modification de la notice
20/08/2019 15:19
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