Monophyly of beta-tubulin and H+-ATPase gene variants in Glomus intraradices: consequences for molecular evolutionary studies of AM fungal genes

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Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_AEFB737D824B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Monophyly of beta-tubulin and H+-ATPase gene variants in Glomus intraradices: consequences for molecular evolutionary studies of AM fungal genes
Périodique
Fungal Genetics and Biology
Auteur⸱e⸱s
Corradi  N., Kuhn  G., Sanders  I. R.
Statut éditorial
Publié
Date de publication
2004
Volume
41
Numéro
2
Pages
262-273
Notes
764QJ FUNGAL GENET BIOL
Résumé
Arbuscular mycorrhizal fungi (AMF) are an ecologically important group of fungi. Previous studies showed the presence of divergent copies of beta-tubulin and V-type vacuolar H+- ATPase genes in AMF genomes and suggested horizontal gene transfer from host plants or mycoparasites to AMF. We sequenced these genes from DNA isolated from an in vitro cultured isolate of Glomus intraradices that was free of any obvious contaminants. We found two highly variable beta-tubulin sequences and variable H+-ATPase sequences. Despite this high variation comparison of the sequences with those in gene banks supported a glomeromycotan origin of G. intraradices beta- tubulin and H+-ATPase sequences. Thus our results are in sharp contrast with the previously reported polyphyletic origin of those genes. We present evidence that some highly divergent sequences of beta-tubulin and H+-ATPase deposited in the databases are likely to be contaminants. We therefore reject the prediction of horizontal transfer to AMF genomes. High differences in GC content between glomeromycotan sequences and sequences grouping in other lineages are shown and we suggest they can be used as an indicator to detect such contaminants. H+-ATPase phylogeny gave unexpected results and failed to resolve fungi as a natural group. beta-Tubulin phylogeny supported Glomeromeromycota as sister group of the Chytridiomycota. Contrasts between our results and trees previously generated using rDNA sequences are discussed. (C) 2004 Elsevier Inc. All rights reserved.
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19/11/2007 11:44
Dernière modification de la notice
20/08/2019 16:18
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