Regional distribution of neuropeptide Y-like immunoreactivity in human hypothalamus measured by immunoradiometric assay: possible influence of chronic respiratory failure on tissue levels
Détails
ID Serval
serval:BIB_AEC664337587
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Regional distribution of neuropeptide Y-like immunoreactivity in human hypothalamus measured by immunoradiometric assay: possible influence of chronic respiratory failure on tissue levels
Périodique
Neuroendocrinology
ISSN
0028-3835 (Print)
Statut éditorial
Publié
Date de publication
01/1990
Volume
51
Numéro
1
Pages
23-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
The regional distribution of neuropeptide Y-like immunoreactivity (NPY-IR) in the human hypothalamus has been determined using a highly specific immunoradiometric assay. Hypothalami were removed during postmortem examination from 19 subjects. The pituitary stalk and 11 anatomically defined nuclei and areas were microdissected from one or both sides of each hypothalamus. NPY-IR was detectable in the acid extracts of tissue samples prepared from all the hypothalamic regions studied, with the highest concentrations being found in the infundibular nucleus (325 +/- 53 fmol/mg wet weight of tissue) and the ventromedial nucleus (217 +/- 22 fmol/mg). For the 11 subjects where both sides of the hypothalamus were dissected, values obtained for the areas in one half showed a good degree of symmetry with the corresponding areas on the contralateral side. The infundibular nucleus exhibited the greatest range of values (72-1,137 fmol/mg). Interestingly, variations in other parts of the hypothalamus were observed to parallel those of this nucleus. Expressed as correlation coefficients (r), levels in the infundibular nucleus appeared to be most closely related to those of the ventromedial nucleus (VM; r = 0.89) and paraventricular nucleus (PV; r = 0.84). In addition, retrospective analysis of the clinical histories showed that all patients with very high NPY levels in the infundibular nucleus (621.0 +/- 107.7 fmol/mg; n = 8) had suffered from respiratory failure or severe dyspnea of at least 10 days duration prior to death. The remaining patients (166.7 +/- 17.1 fmol/mg; n = 11) had either died 48 h from the onset of cardiorespiratory difficulties or of unrelated causes.(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Aged
Aged, 80 and over
Chronic Disease
Female
Humans
Hypothalamus/*metabolism
Immunoradiometric Assay
Male
Middle Aged
Neuropeptide Y/*metabolism
Respiratory Insufficiency/*metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 17:26
Dernière modification de la notice
20/08/2019 16:18