Vasopressin and angiotensin induce inositol lipid breakdown in rat adenohypophysial cells in primary culture

Détails

ID Serval
serval:BIB_AE878CA03BC6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Vasopressin and angiotensin induce inositol lipid breakdown in rat adenohypophysial cells in primary culture
Périodique
Regulatory Peptides
Auteur⸱e⸱s
Guillon  G., Gaillard  R. C., Kehrer  P., Schoenenberg  P., Muller  A. F., Jard  S.
ISSN
0167-0115 (Print)
Statut éditorial
Publié
Date de publication
08/1987
Volume
18
Numéro
3-4
Pages
119-29
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 17
Résumé
Adenohypophysial cells from female Wistar rats were dispersed and maintained for 4 days in primary culture in the presence of [3H]myoinositol. The effects of several releasing hormones, corticotropin-releasing factor (CRF), arginine vasopressin (AVP), angiotensin II (A II), thyrotropin-releasing hormone (TRH), and luteinizing hormone-releasing hormone (LHRH) on the liberation of labelled inositol phosphate (InsP), inositol-bisphosphate (InsP2), and inositol-trisphosphate (InsP3) from prelabelled inositol lipids were tested alone and in combination. Of the corticotropin (ACTH) secretagogues tested, AVP and A II produced a dose-dependent increase in inositol phosphate accumulation. CRF was inactive. The ED50 values of about 1 nM for both AVP and A II were close to the corresponding dissociation constants for binding to pituitary membranes: and, in the case of A II, close to the ED50 for A II-induced inhibition of pituitary membrane adenylate cyclase. The responses to A II and AVP could be inhibited by [Sar1,Ile8]A II and the AVP antagonist d(Et2)-VAVP, respectively. The magnitude of the maximal effect of AVP on accumulation of inositol phosphates was small (25% increase over basal value) suggesting that this effect was restricted to a minor subpopulation of pituitary cells (probably corticotrophes). CRF did not potentiate AVP-induced inositol phosphates accumulation. Maximal A II-induced increase in inositol phosphates accumulation represented 150% of the basal value and was partially additive with that of TRH suggesting that lactotrophes represent the main A II-sensitive subpopulation.
Mots-clé
Adrenocorticotropic Hormone/metabolism Angiotensin II/*pharmacology Animals Arginine Vasopressin/*pharmacology Cells, Cultured Chromatography, Ion Exchange Corticotropin-Releasing Hormone/pharmacology Cyclic AMP/metabolism Dose-Response Relationship, Drug Female Inositol/*metabolism Inositol Phosphates/metabolism *Lipid Mobilization Pituitary Gland, Anterior/*metabolism Radioimmunoassay Rats Thyrotropin-Releasing Hormone/pharmacology Time Factors
Pubmed
Web of science
Création de la notice
15/02/2008 17:57
Dernière modification de la notice
20/08/2019 16:18
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