Contribution of (sub)domains of Staphylococcus aureus fibronectin-binding protein to the proinflammatory and procoagulant response of human vascular endothelial cells.

Détails

ID Serval
serval:BIB_AE718F07FC46
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Contribution of (sub)domains of Staphylococcus aureus fibronectin-binding protein to the proinflammatory and procoagulant response of human vascular endothelial cells.
Périodique
Thrombosis and Haemostasis
Auteur⸱e⸱s
Heying R., van de Gevel J., Que Y.A., Piroth L., Moreillon P., Beekhuizen H.
ISSN
0340-6245
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
101
Numéro
3
Pages
495-504
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
The Staphylococcus aureus fibronectin (Fn) -binding protein A (FnBPA) is involved in bacterium-endothelium interactions which is one of the crucial events leading to infective endocarditis (IE). We previously showed that the sole expression of S. aureus FnBPA was sufficient to confer to non-invasive Lactococcus lactis bacteria the capacity to invade human endothelial cells (ECs) and to launch the typical endothelial proinflammatory and procoagulant responses that characterize IE. In the present study we further questioned whether these bacterium-EC interactions could be reproduced by single or combined FnBPA sub-domains (A, B, C or D) using a large library of truncated FnBPA constructs expressed in L. lactis. Significant invasion of cultured ECs was found for L. lactis expressing the FnBPA subdomains CD (aa 604-877) or A4(+16) (aa 432-559). Moreover, this correlates with the capacity of these fragments to elicit in vitro a marked increase in EC surface expression of both ICAM-1 and VCAM-1 and secretion of the CXCL8 chemokine and finally to induce a tissue factor-dependent endothelial coagulation response. We thus conclude that (sub)domains of the staphylococcal FnBPA molecule that express Fn-binding modules, alone or in combination, are sufficient to evoke an endothelial proinflammatory as well as a procoagulant response and thus account for IE severity.
Mots-clé
Adhesins, Bacterial/genetics, Adhesins, Bacterial/immunology, Cells, Cultured, Endothelial Cells/immunology, Endothelial Cells/metabolism, Fibronectins/metabolism, Humans, Lactococcus lactis/genetics, Mutant Proteins/genetics, Mutant Proteins/immunology, Staphylococcal Infections/genetics, Staphylococcal Infections/immunology, Staphylococcus aureus/genetics, Staphylococcus aureus/immunology, Thromboplastin/metabolism
Pubmed
Web of science
Création de la notice
03/02/2010 16:47
Dernière modification de la notice
20/08/2019 15:18
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