New comprehension of the apicoplast of Sarcocystis by transmission electron tomography.

Détails

ID Serval
serval:BIB_AE6600BBF610
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
New comprehension of the apicoplast of Sarcocystis by transmission electron tomography.
Périodique
Biology of the Cell
Auteur⸱e⸱s
Tomova C., Geerts W.J.C., Müller-Reichert T., Entzeroth R., Humbel B.M.
ISSN
0248-4900 (Print)
ISSN-L
0248-4900
Statut éditorial
Publié
Date de publication
2006
Volume
98
Numéro
9
Pages
535-545
Langue
anglais
Résumé
BACKGROUND INFORMATION: Apicomplexan parasites (like Plasmodium, Toxoplasma, Eimeria and Sarcocystis) contain a distinctive organelle, the apicoplast, acquired by a secondary endosymbiotic process analogous to chloroplasts and mitochondria. The apicoplast is essential for long-term survival of the parasite. This prokaryotic origin implies that molecular and metabolic processes in the apicoplast differ from those of the eukaryotic host cells and therefore offer options for specific chemotherapeutic treatment. We studied the apicoplast in high-pressure frozen and freeze-substituted cysts of Sarcocystis sp. from roe deer (Capreolus capreolus) to get better insight in apicoplast morphology. RESULTS AND CONCLUSIONS: We observed that the apicoplast contains four continuous membranes. The two inner membranes have a circular shape with a constant distance from each other and large-sized protein complexes are located between them. The two outer membranes have irregular shapes. The periplastid membrane also contains large-sized protein complexes, while the outer membrane displays protuberances into the parasite cytoplasm. In addition, it is closely associated with the endoplasmic reticulum by 'contact sites'.
Mots-clé
Animals, Deer/parasitology, Intracellular Membranes/metabolism, Intracellular Membranes/ultrastructure, Microscopy, Electron, Transmission/methods, Multiprotein Complexes/metabolism, Multiprotein Complexes/ultrastructure, Organelles/metabolism, Organelles/ultrastructure, Protozoan Proteins/metabolism, Protozoan Proteins/ultrastructure, Sarcocystis/metabolism, Sarcocystis/ultrastructure, Tomography, X-Ray Computed/methods
Pubmed
Web of science
Création de la notice
28/02/2012 19:45
Dernière modification de la notice
20/08/2019 16:18
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