Urinary enzyme excretion and changes in renal functions induced by toxic substances or by renal ischemia in rats

Détails

ID Serval
serval:BIB_AD66BCC6E1AB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Urinary enzyme excretion and changes in renal functions induced by toxic substances or by renal ischemia in rats
Périodique
Archives of Toxicology. Supplement
Auteur(s)
Ansermet  F., Mieville  C., Diezi  J.
ISSN
0171-9750
Statut éditorial
Publié
Date de publication
1980
Volume
4
Pages
201-7
Notes
Journal Article
Résumé
The urinary excretion of four enzymes (alkaline phosphatase: AP, leucine aminopeptidase: LAP, lactate dehydrogenase: LDH, muramidase: M) was measured in unanesthetized adult male Wistar rats within 48 h after either a single injection of mercuric chloride (HgCl2) (0.5-1.0 mg x kg-1), or of gentamicin (2.5-25 mg x kg-1), or of tobramoycin (2.5-25 mg x kg-1), or after 30 min of clamping of both renal arteries. Glomerular filtration rate (GFR), TmPAH, plasma urea, urinary protein and sodium excretion were measured simultaneously. The excretion of AP, LAP and LDH, but not that of M, increased significantly above control levels after renal ischemia or the nephrotoxic agents; the increase was dose-related after HgCl2. GFR was not depressed, but TmPAH decreased after the higher doses of the toxic agents. Though more sensitive for detecting minor grades of acute renal damage than function tests, measurements of urinary enzyme excretion were fraught with large inter-individual variation, and variable time-course of changes in different types of renal damage. Short-term exposure (3 months) to phenylmercuric acetate was associated with a significant decrease of the urinary excretion of AP, and of LAP, and of AP activity measured histochemically in proximal tubular cells.
Mots-clé
Acute Disease Animals Enzymes/*urine Histocytochemistry Ischemia/*enzymology Kidney/*blood supply Kidney Diseases/chemically induced/*enzymology Male Rats Regional Blood Flow
Pubmed
Création de la notice
21/01/2008 12:54
Dernière modification de la notice
20/08/2019 16:17
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