The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_ACD96D382E77
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis.
Périodique
Journal of Cell Biology
Auteur(s)
Maerki S., Olma M.H., Staubli T., Steigemann P., Gerlich D.W., Quadroni M., Sumara I., Peter M.
ISSN
1540-8140[electronic], 0021-9525[linking]
Statut éditorial
Publié
Date de publication
2009
Volume
187
Numéro
6
Pages
791-800
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Cul3 (Cullin3)-based E3 ubiquitin ligases recently emerged as critical regulators of mitosis. In this study, we identify two mammalian BTB (Bric-a-brac-Tramtrack-Broad complex)-Kelch proteins, KLHL21 and KLHL22, that interact with Cul3 and are required for efficient chromosome alignment. Interestingly, KLHL21 but not KLHL22 is necessary for cytokinesis and regulates translocation of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase, similar to the previously described BTB-Kelch proteins KLHL9 and KLHL13. KLHL21 directly binds to aurora B and mediates ubiquitination of aurora B in vitro. In contrast to KLHL9 and KLHL13, KLHL21 localizes to midzone microtubules in anaphase and recruits aurora B and Cul3 to this region. Together, our results suggest that different Cul3 adaptors nonredundantly regulate aurora B during mitosis, possibly by ubiquitinating different pools of aurora B at distinct subcellular localizations.
Mots-clé
Anaphase/genetics, Chromosome Segregation, Cullin Proteins/genetics, Cullin Proteins/metabolism, Cytokinesis/genetics, Hela Cells, Humans, Microtubules/enzymology, Protein Processing, Post-Translational, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, RNA Interference, Recombinant Fusion Proteins/metabolism, Time Factors, Transfection, Tubulin/metabolism, Ubiquitin-Protein Ligases/genetics, Ubiquitin-Protein Ligases/metabolism, Ubiquitination
Pubmed
Web of science
Open Access
Oui
Création de la notice
05/01/2010 15:19
Dernière modification de la notice
20/08/2019 16:16
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