Determination of cardiac contractility in awake unsedated mice with a fluid-filled catheter.

Détails

ID Serval
serval:BIB_ACD019DA9769
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Determination of cardiac contractility in awake unsedated mice with a fluid-filled catheter.
Périodique
American Journal of Physiology. Heart and Circulatory Physiology
Auteur⸱e⸱s
Wang Q., Brunner H.R., Burnier M.
ISSN
0363-6135
Statut éditorial
Publié
Date de publication
02/2004
Peer-reviewed
Oui
Volume
286
Numéro
2
Pages
H806-814
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Today, cardiac contractility in mice is exclusively measured under anesthesia or in sedated animals because the catheters available are too rigid to be used in awake mice. We therefore developed a new catheter (Pebax 03) to measure cardiac contractility in conscious mice. In this study, we evaluated the accuracy and utility of this new catheter for assessment of cardiac contractility in anesthetized and conscious mice. With the use of a balloon-pop test, the Pebax catheter with an inner diameter of 0.3 mm was found to exhibit a high natural frequency, a low damping coefficient, and a flat frequency of up to 50.5 +/- 0.6 Hz. Under anesthesia (0.5% or 1.0% halothane), no difference was found in heart rate (HR), left ventricular (LV) systolic pressure (LVSP), the maximum rates of LV pressure rise and fall (LV dP/dt(max) and LV dP/dt(min), respectively), ejection time (ET), and isovolumic relaxation time constant (tau) when measured with either the 1.4-Fr Millar or Pebax 03 catheter. However, when HR, LVSP, LV dP/dt(max), and LV dP/dt(min) were recorded with the Pebax catheter in awake mice, values were significantly higher, and ET and tau were lower, than under anesthesia, suggesting a major impact of anesthesia on these parameters. The Pebax catheter was also used in a normotensive one-renin gene mouse model of cardiac hypertrophy induced by DOCA and salt. In this model, DOCA-salt induced a severe decrease in cardiac contractility in the absence of changes in blood pressure. These data demonstrate that cardiac contractility can be measured very accurately in conscious mice. This new device can be of great help in the investigation of cardiac function in normal and genetically engineered mice.
Mots-clé
Anesthesia, General, Animals, Catheterization, Desoxycorticosterone/pharmacology, Hemodynamics/drug effects, Hemodynamics/physiology, Male, Mice, Mice, Inbred C57BL, Myocardial Contraction/physiology, Nephrectomy, Renin/genetics, Ventricular Function, Left/physiology, Wakefulness/physiology
Pubmed
Web of science
Création de la notice
25/01/2008 12:55
Dernière modification de la notice
20/08/2019 15:16
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