Poker players are at high risk of experiencing gambling problems. Despite the feminization of gambling, little is known about the problems associated with poker playing among women. This cross-sectional study aims to examine relationships between gambling problems and factors generally associated with gambling problems (gambling behaviours, substance use and mental health) among women poker players. A total of 46 women were recruited through a broader prospective cohort study from the province of Quebec, Canada. The outcome variable of interest was participants' scores on the Problem Gambling Severity Index (PGSI); the predictive variables were gambling behaviours, alcohol and drug misuse (DEBAAlcohol and drugs) and mental health (Beck anxiety and depression). Multiple logistic regression analyses revealed that the factors associated with gambling problems among women poker players are distinct from those of men. For women, severity of gambling problems is positively associated with Internet poker playing, playing slot machines and smoking cigarettes (p <.05). However, among these women, alcohol and drug misuse and mental health are not associated with gambling problems. Therefore, it is important to understand the risks associated with women poker players to develop preventive strategies adapted for this population and to adjust interventions accordingly.