Coexpression and Copurification of RNA–Protein Complexes in Escherichia coli
Détails
ID Serval
serval:BIB_ACACE445B980
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Institution
Titre
Coexpression and Copurification of RNA–Protein Complexes in Escherichia coli
Titre du livre
RNA Scaffolds
Editeur
Springer US
ISBN
9781071614983
9781071614990
9781071614990
ISSN
1064-3745
1940-6029
1940-6029
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2021
Peer-reviewed
Oui
Volume
2323
Numéro de chapitre
6
Pages
67-73
Langue
anglais
Résumé
For structural, biochemical, or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows for efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA-protein coexpression in order to express and purify RNA by affinity in native condition. As a proof of concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
Pubmed
Web of science
Création de la notice
15/06/2021 15:13
Dernière modification de la notice
29/07/2024 8:40