Coexpression and Copurification of RNA–Protein Complexes in Escherichia coli

Détails

ID Serval
serval:BIB_ACACE445B980
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Titre
Coexpression and Copurification of RNA–Protein Complexes in Escherichia coli
Titre du livre
RNA Scaffolds
Auteur⸱e⸱s
El Khouri Margot, Catala Marjorie, Seijo Bili, Chabal Johana, Dardel Frédéric, Tisné Carine, Ponchon Luc
Editeur
Springer US
ISBN
9781071614983
9781071614990
ISSN
1064-3745
1940-6029
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2021
Peer-reviewed
Oui
Volume
2323
Numéro de chapitre
6
Pages
67-73
Langue
anglais
Résumé
For structural, biochemical, or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows for efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA-protein coexpression in order to express and purify RNA by affinity in native condition. As a proof of concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
Pubmed
Web of science
Création de la notice
15/06/2021 15:13
Dernière modification de la notice
29/07/2024 8:40
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