Developmental and regional changes in the neurochemical profile of the rat brain determined by in vivo 1H NMR spectroscopy.

Détails

ID Serval
serval:BIB_AC65F7CCEE63
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Developmental and regional changes in the neurochemical profile of the rat brain determined by in vivo 1H NMR spectroscopy.
Périodique
Magnetic Resonance in Medicine
Auteur⸱e⸱s
Tkác I., Rao R., Georgieff M.K., Gruetter R.
ISSN
0740-3194 (Print)
ISSN-L
0740-3194
Statut éditorial
Publié
Date de publication
2003
Volume
50
Numéro
1
Pages
24-32
Langue
anglais
Notes
Publication types: Comparative Study ; Evaluation Studies ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. ; Validation StudiesPublication Status: ppublish
Résumé
Sixteen metabolites were quantified from 11-24 micro l volumes in three different brain regions (hippocampus, striatum, and cerebral cortex) during postnatal development. Rat pups from the same litter were repeatedly measured on postnatal days 7, 10, 14, 21, and 28 using a completely noninvasive and longitudinal study design. Metabolite quantification was based on ultra-short echo-time (1)H NMR spectroscopy at 9.4 T and LCModel processing. Most of the brain metabolites were quantified with Cramer-Rao lower bounds (CRLB) less than 20%, which corresponded to an estimated concentration error <0.2 micro mol/g. Taurine and total creatine were quantified with CRLB < or = 5% from all 114 processed spectra. The resulting high reliability and reproducibility revealed significant regional and age-related changes in metabolite concentrations. The most sensitive markers for developmental and regional variations between hippocampus, striatum, and cerebral cortex were N-acetylaspartate, myo-inositol, taurine, glutamate, and choline compounds. Absolute values of metabolite concentrations were in very good agreement with previously published in vitro results based on chromatographic measurements of brain extracts. The current data may serve as a reference for studies focused on developmental defects and pathologies using neonatal rat models.
Mots-clé
Aging/physiology, Animals, Animals, Newborn, Brain Chemistry/physiology, Brain Mapping/methods, Cerebellar Cortex/metabolism, Corpus Striatum/metabolism, Energy Metabolism/physiology, Hippocampus/metabolism, Magnetic Resonance Spectroscopy/methods, Neurons/metabolism, Protons, Rats, Rats, Sprague-Dawley, Tissue Distribution
Pubmed
Web of science
Open Access
Oui
Création de la notice
04/08/2010 16:28
Dernière modification de la notice
20/08/2019 16:16
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