Localization of epithelial sodium channel and aquaporin-2 in rabbit kidney cortex

Détails

ID Serval
serval:BIB_AC4AB35ED1EC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Localization of epithelial sodium channel and aquaporin-2 in rabbit kidney cortex
Périodique
American Journal of Physiology. Renal Physiology
Auteur(s)
Loffing  J., Loffing-Cueni  D., Macher  A., Hebert  S. C., Olson  B., Knepper  M. A., Rossier  B. C., Kaissling  B.
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
04/2000
Peer-reviewed
Oui
Volume
278
Numéro
4
Pages
F530-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
The amiloride-sensitive epithelial sodium channel (ENaC) and the vasopressin-dependent water channel aquaporin-2 (AQP2) mediate mineralocorticoid-regulated sodium- and vasopressin-regulated water reabsorption, respectively. Distributions of ENaC and AQP2 have been shown by immunohistochemistry in rats. Functional data from rabbits suggest a different distribution pattern of these channels than in rats. We studied, by immunohistochemistry in the rabbit kidney cortex, the distributions of ENaC and AQP2, in conjunction with marker proteins for distal segments. In rabbit cortex ENaC is restricted to the connecting tubule (CNT) cells and cortical collecting duct (CCD) cells. The intracellular distribution of ENaC shifts from the apical membrane in the most upstream CNT cells to a cytoplasmic location further downstream in the CNT and in the CCD cells. AQP2 is detected in the CCD cells exclusively. The anatomic subdivisions in the rabbit distal nephron coincide exactly with distributions of apical transport systems. The differences between rabbits and rats in the distribution patterns of ENaC and AQP2 may explain functional differences in renal salt and water handling between these species.
Mots-clé
Animals Aquaporin 2 Aquaporin 6 Aquaporins/*metabolism Benzothiadiazines Bumetanide/pharmacology Calcium-Binding Protein, Vitamin D-Dependent/metabolism Carrier Proteins/*drug effects/metabolism Diuretics/pharmacology Epithelial Sodium Channel Female Kidney Cortex/anatomy & histology/*metabolism Male Proton-Translocating ATPases/metabolism Rabbits Rats Sodium Channels/*metabolism Sodium Chloride Symporter Inhibitors/pharmacology Sodium-Potassium-Chloride Symporters Tissue Distribution
Pubmed
Web of science
Création de la notice
24/01/2008 13:00
Dernière modification de la notice
20/08/2019 15:16
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