Growth and metabolic characteristics of fastidious meat-derived Lactobacillus algidus strains.
Détails
ID Serval
serval:BIB_ABD2FA9BE9FF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Growth and metabolic characteristics of fastidious meat-derived Lactobacillus algidus strains.
Périodique
International journal of food microbiology
ISSN
1879-3460 (Electronic)
ISSN-L
0168-1605
Statut éditorial
Publié
Date de publication
16/01/2020
Peer-reviewed
Oui
Volume
313
Pages
108379
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Lactobacillus algidus is a meat spoilage bacterium often dominating the bacterial communities on chilled, packaged meat. Yet, L. algidus strains are rarely recovered from meat, and only few studies have focused on this species. The main reason limiting detailed studies on L. algidus is related to its poor growth on the media routinely used for culturing food spoilage bacteria. Thus, our study sought to develop reliable culture media for L. algidus to enable its recovery from meat, and to allow subculturing and phenotypic analyses of the strains. We assessed the growth of meat-derived L. algidus strains on common culture media and their modifications, and explored the suitability of potential media for the recovery of L. algidus from meat. Moreover, we determined whether 12 meat-derived L. algidus strains selected from our culture collection produce biogenic amines that may compromise safety or quality of meat, and finally, sequenced de novo and annotated the genomes of two meat-derived L. algidus strains to uncover genes and metabolic pathways relevant for phenotypic traits observed. MRS agar supplemented with complex substances (peptone, meat and yeast extract, liver digest) supported the growth of L. algidus, and allowed the recovery of new L. algidus isolates from meat. However, most strains grew poorly on standard MRS agar and on general-purpose media. In MRS broth, most strains grew well but a subset of strains required supplementation of MRS broth with additional cysteine. Supplementation of MRS broth with catalase allowed growth in aerated cultures suggesting that the strains produced hydrogen peroxide when grown aerobically. The strains tested (n = 12) produced ornithine from arginine and putrescine from agmatine, and two strains produced tyramine from tyrosine. Our findings reveal that L. algidus populations are underestimated if routine culture protocols are applied, and prompt concerns that L. algidus may generate tyramine or putrescine in meat or fermented meat products.
Mots-clé
Animals, Biogenic Amines/analysis, Biogenic Amines/metabolism, Cattle, Culture Media/metabolism, Fermentation, Lactobacillus/growth & development, Lactobacillus/metabolism, Meat Products/analysis, Meat Products/microbiology, Putrescine/analysis, Putrescine/metabolism, Swine, Biogenic amines, Lactobacillus algidus, MRS medium, Meat spoilage, Putrescine, Tyramine
Pubmed
Web of science
Création de la notice
16/12/2019 12:37
Dernière modification de la notice
10/02/2024 7:14