Implication of the exon region in the regulation of the human telomerase reverse transcriptase gene promoter

Détails

ID Serval
serval:BIB_ABCD8EF9DDF6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Implication of the exon region in the regulation of the human telomerase reverse transcriptase gene promoter
Périodique
Biochemical and Biophysical Research Communications
Auteur⸱e⸱s
Renaud  S., Bosman  F. T., Benhattar  J.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
2003
Volume
300
Numéro
1
Pages
47-54
Notes
PT - Journal Article PT - Research Support, Non-U.S. Gov't
Résumé
The expression of the catalytic subunit (hTERT) represents the limiting factor for telomerase activity. In transfection studies, high level of activity of hTERT promoter is found, whereas low copy numbers of hTERT mRNA are detected in vivo. To explain this discrepancy, a series of vectors containing the hTERT promoter and gene were transiently transfected into HeLa cells. Four important regions were identified. First, the core promoter has bidirectional activity. Second, the distal upstream region (-1821 to -811bp) involved in the splicing of the first intron and could be a key of splicing specificity. Third, the intermediate promoter region (-800 to -300bp) could play an important role in silencing the reverse promoter activity. Fourth, the structural gene (up to +1077) strongly reduced hTERT promoter activity. These results provide the first evidence that the first two exons play a major role in the down-regulation of the hTERT promoter in telomerase-positive cells
Mots-clé
Codon,Initiator/genetics/DNA-Binding Proteins/Down-Regulation/Exons/Gene Expression Regulation,Enzymologic/Hela Cells/Humans/Promoter Regions (Genetics)/RNA Splicing/Reading Frames/Telomerase/Transfection
Pubmed
Web of science
Création de la notice
29/01/2008 19:35
Dernière modification de la notice
20/08/2019 16:15
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