Comparison of phorbol-12-myristate-13-acetate and dioctanoyl-sn-glycerol in the activation of EL4/6.1 thymoma cells

Détails

ID Serval
serval:BIB_ABCBC5EAED55
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparison of phorbol-12-myristate-13-acetate and dioctanoyl-sn-glycerol in the activation of EL4/6.1 thymoma cells
Périodique
Journal of Cellular Physiology
Auteur(s)
Stoeck  M., Lees  R., Szamel  M., Pantaleo  G., MacDonald  H. R.
ISSN
0021-9541 (Print)
Statut éditorial
Publié
Date de publication
03/1989
Volume
138
Numéro
3
Pages
541-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
The present study compared the role of two protein kinase C (PK-C) activating agents, the phorbol ester phorbol-12-acetate-13-myristate (PMA) and the membrane-permeating diacylglycerol dioctanoyl-sn-glycerol (DiC8) in the activation of EL4/6.1 thymoma cells. These cells have been shown to express interleukin-2 receptors (IL-2R) upon stimulation with optimal amounts of PMA (10 ng/ml); also, suboptimal amounts of PMA (1 ng/ml) synergized with the Ca2+ ionophore ionomycin and recombinant interleukin-1 (rIL-1) (Lowenthal et al., 1986). Comparing PMA and DiC8 led to the following results: PMA at 10 ng/ml induced IL-2R; in contrast, DiC8 (30-3 micrograms/ml) alone was unable to induce IL-2R, although it did synergize with ionomycin (0.5 micrograms/ml) and rIL-1. Bihourly additions of DiC8 did not change this pattern. The addition of DiC8 together with rIL-2 also resulted in no IL-2R expression. Furthermore, DiC8 (10 micrograms/ml) effectively translocated PK-C. Therefore, the differences observed between PMA and DiC8 do not seem to be due to differences in metabolism or to an inability to translocate PK-C. Analysis of messenger (m) RNA produced in stimulated EL4/6.1 cells revealed that DiC8 was also unable to induce mRNA for IL-2R. Our data suggest that PMA, especially at "optimal" concentrations, might have effects that cannot be mimicked by diacylglycerol. Furthermore, it seems that the deficient activity of diacylglycerols can be compensated for by a Ca2+ ionophore and, depending on the cellular system, by further signals such as IL-1.
Mots-clé
Animals Diglycerides/*pharmacology Ethers/pharmacology Flow Cytometry Glycerides/*pharmacology Interleukin-1/pharmacology Ionomycin Lymphocyte Activation/*drug effects Mice Protein Kinase C/metabolism RNA, Messenger/genetics Receptors, Interleukin-2/biosynthesis/genetics Tetradecanoylphorbol Acetate/*pharmacology Thymoma/*pathology Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
25/01/2008 16:14
Dernière modification de la notice
20/08/2019 16:15
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