Inducible cardiac-restricted expression of enteroviral protease 2A is sufficient to induce dilated cardiomyopathy

Détails

ID Serval
serval:BIB_AB5CCFD325E4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Inducible cardiac-restricted expression of enteroviral protease 2A is sufficient to induce dilated cardiomyopathy
Périodique
Circulation
Auteur(s)
Xiong  D., Yajima  T., Lim  B. K., Stenbit  A., Dublin  A., Dalton  N. D., Summers-Torres  D., Molkentin  J. D., Duplain  H., Wessely  R., Chen  J., Knowlton  K. U.
ISSN
1524-4539 (Electronic)
Statut éditorial
Publié
Date de publication
01/2007
Volume
115
Numéro
1
Pages
94-102
Notes
Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't --- Old month value: Jan 2
Résumé
BACKGROUND: Enterovirus infection is a cause of cardiomyopathy. We previously demonstrated that enteroviral protease 2A directly cleaves the cytoskeletal protein dystrophin. However, the direct effect of protease 2A in enteroviral cardiomyopathy is less clear because other viral proteins are also expressed with viral infection. METHODS AND RESULTS: A transgenic mouse with inducible cardiac-restricted expression of enteroviral protease 2A was generated. In the transgenic mouse, a tamoxifen-regulated Cre-loxP system, MerCreMer (MCM), was used to induce genetic recombination in cardiac myocytes, which led to protease 2A expression. Protease 2A and MCM double transgenic (2AxMCM) mice were treated with tamoxifen; the controls included 2AxMCM mice treated with diluents for tamoxifen and tamoxifen-treated MCM littermates. Protease 2A activity was significantly induced after tamoxifen in the 2AxMCM mice compared with controls. Echocardiographic analysis demonstrated an increase in left ventricular end-diastolic and end-systolic chamber size, with decreased fractional shortening in tamoxifen-treated 2AxMCM mice. There was an increase in heart weight-to-body weight ratio in 2AxMCM mice treated with tamoxifen. Only a small increase in interstitial fibrosis and inflammation was found in tamoxifen-treated 2AxMCM mice; however, ultrastructural analysis demonstrated myofibrillar collapse with abnormalities of intercalated discs and sarcolemmal membranes. Evans blue dye-positive myocytes with disruption of dystrophin were present in 2AxMCM mice treated with tamoxifen. Disruption of dystrophin was also found in cultured myocytes isolated from 2AxMCM mice with Cre in the nucleus. CONCLUSIONS: Protease 2A has a significant role in enteroviral cardiomyopathy and alone is sufficient to induce dilated cardiomyopathy, which is associated with disruption of the sarcolemmal membrane and cleavage of dystrophin with protease 2A expression.
Mots-clé
Animals Cardiomyopathy, Dilated/*enzymology/genetics/virology Cysteine Endopeptidases/*biosynthesis/genetics Enterovirus/*enzymology/genetics Gene Expression Regulation, Enzymologic/physiology Male Mice Mice, Inbred BALB C Mice, Inbred C3H Mice, Inbred C57BL Mice, Transgenic Myocytes, Cardiac/*enzymology Viral Proteins/*biosynthesis/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:44
Dernière modification de la notice
20/08/2019 16:15
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