DNA segments mapped by reciprocal use of microsatellite primers between mouse and rat

Détails

ID Serval
serval:BIB_AA24E9A87E38
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
DNA segments mapped by reciprocal use of microsatellite primers between mouse and rat
Périodique
Mammalian Genome
Auteur⸱e⸱s
Kondo  Y., Mori  M., Kuramoto  T., Yamada  J., Beckmann  J. S., Simon-Chazottes  D., Montagutelli  X., Guenet  J. L., Serikawa  T.
ISSN
0938-8990 (Print)
Statut éditorial
Publié
Date de publication
1993
Volume
4
Numéro
10
Pages
571-6
Notes
Journal Article
Research Support, Non-U.S. Gov't
Résumé
Rat microsatellite primers were used for detection of homologous DNA segments in the mouse species (Mus laboratorius, Mus musculus musculus, and Mus spretus). Twenty five (16.3%) of 153 rat primer pairs amplified specific DNA segments, when genomic DNA of mice was used as a template in the polymerase chain reaction (PCR). Size variation among inbred strains of mice was found for 13 DNA segments (8.5%). Eight out of the 13 polymorphic DNA segments were mapped to a particular chromosome with two sets of recombinant inbred strains, AKXL or BXD. Similarly, mouse microsatellite primers were used for detection of homologous DNA segments in rats (Rattus norvegicus). Twenty (12.0%) of 166 primer pairs amplified specific DNA segments from rat genome. Size variation among inbred strains of rats was found for seven DNA segments (4.2%). Eleven of these 20 DNA segments were mapped with a rat x mouse somatic cell hybrid clone panel and/or linkage analysis by use of backcross progeny. Our results suggest that the mapped DNA segments are really homologs between mouse and rat. These polymorphic DNA segments are useful genetic markers.
Mots-clé
Animals Base Sequence Chromosome Mapping/*methods Crosses, Genetic *DNA Primers *DNA, Satellite Female Genetic Markers Male Mice Mice, Inbred Strains Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Rats Rats, Inbred Strains Sequence Homology, Nucleic Acid
Pubmed
Web of science
Création de la notice
25/01/2008 16:17
Dernière modification de la notice
20/08/2019 15:14
Données d'usage