Differential subcellular localization of ENaC subunits in mouse kidney in response to high- and low-Na diets
Détails
ID Serval
serval:BIB_A99E492EB1C4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Differential subcellular localization of ENaC subunits in mouse kidney in response to high- and low-Na diets
Périodique
American Journal of Physiology. Renal Physiology
ISSN
0363-6127
Statut éditorial
Publié
Date de publication
08/2000
Peer-reviewed
Oui
Volume
279
Numéro
2
Pages
F252-8
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug
Résumé
Previous electrophysiological experiments on renal cortical collecting ducts indicated that dietary sodium intake and variations in aldosterone plasma levels regulate the abundance of functional epithelial Na channels (ENaC) in the apical plasma membrane. In mouse kidney we investigated by immunohistochemistry whether feeding for 3 wk a diet with high (3% Na) and low (0.05% Na) Na content influences the distribution pattern of ENaC. In mice of all experimental groups, ENaC was apparent in cells from the late portion of the distal convoluted tubule (DCT2) down to the medullary collecting duct (CD). In mice on a high-Na diet (plasma aldosterone: 40.8 +/- 2.0 ng/dl), the alpha-subunit was undetectable, and the beta- and gamma-ENaC were detected in the cytoplasm, but not in the apical plasma membrane of the cells. In contrast, in mice on a low-Na diet (plasma aldosterone: 93.6 +/- 9.3 ng/dl) all three ENaC subunits were displayed in the subapical cytoplasm and in the apical membrane of DCT2, connecting tubule (CNT), and, although less prominent, in cortical CD cells. Apical plasma membrane immunostaining progressively decreased along the cortical CD, simultaneously with increasing cytoplasmic staining for beta- and gamma-ENaC. Thus our data on mice adapted to moderately low and high Na intake suggest that regulation of ENaC function in vivo involves shifts of beta- and gamma-subunits from the cytoplasm to the apical plasma membrane and vice versa, respectively. The insertion of these subunits into the apical plasma membrane coincides with upregulation of the alpha-subunit and its insertion into the apical plasma membrane.
Mots-clé
Aldosterone/blood
Animals
Cell Membrane/metabolism
*Diet, Sodium-Restricted
Epithelial Sodium Channel
Kidney/*metabolism
Male
Mice
Mice, Inbred C57BL
Natriuresis/physiology
Protein Isoforms/metabolism
Sodium Channels/*metabolism
Sodium, Dietary/pharmacology
Subcellular Fractions/*metabolism
Tissue Distribution
Pubmed
Web of science
Création de la notice
24/01/2008 14:00
Dernière modification de la notice
20/08/2019 16:13