Altered hepatic transport of immunoglobulin A in mice lacking the J chain.

Détails

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Accès restreint UNIL
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_A97DE46A8D0C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Altered hepatic transport of immunoglobulin A in mice lacking the J chain.
Périodique
The Journal of experimental medicine
Auteur⸱e⸱s
Hendrickson B.A., Conner D.A., Ladd D.J., Kendall D., Casanova J.E., Corthesy B., Max E.E., Neutra M.R., Seidman C.E., Seidman J.G.
ISSN
0022-1007
ISSN-L
0022-1007
Statut éditorial
Publié
Date de publication
01/12/1995
Peer-reviewed
Oui
Volume
182
Numéro
6
Pages
1905-1911
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
We have created J chain knockout mice to define the physiologic role of the J chain in immunoglobulin synthesis and transport. The J chain is covalently associated with pentameric immunoglobulin (Ig) M and dimeric IgA and is also expressed in most IgG-secreting cells. J chain-deficient mice have normal serum IgM and IgG levels but markedly elevated serum IgA. Although polymeric IgA was present in the mutant mice, a larger proportion of their serum IgA was monomeric than was found in wild-type mouse serum. Bile and fecal IgA levels were decreased in J chain-deficient mice compared with wild-type mice, suggesting inefficient transport of J chain-deficient IgA by hepatic polymeric immunoglobulin receptors (pIgR). The pIgR-mediated transport of serum-derived IgA from wild-type and mutant mice was assessed in Madin-Darby canine kidney (MDCK) cells transfected with the pIgR. These studies revealed selective transport by pIgR-expressing MDCK cells of wild-type IgA but not J chain-deficient IgA. We conclude that although the J chain is not required for IgA dimerization, it does affect the efficiency of polymerization or have a role in maintaining IgA dimer stability. Furthermore, the J chain is essential for efficient hepatic pIgR transport of IgA.
Mots-clé
Animals, Base Sequence, Bile/metabolism, Biological Transport, Cells, Cultured, DNA Primers/chemistry, Dogs, Genes, Immunoglobulin, Immunoglobulin A/chemistry, Immunoglobulin A/metabolism, Immunoglobulin J-Chains/metabolism, Liver/metabolism, Macromolecular Substances, Mice, Mice, Knockout, Molecular Sequence Data, Restriction Mapping
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 14:53
Dernière modification de la notice
09/08/2024 14:51
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