Altered hepatic transport of immunoglobulin A in mice lacking the J chain
Détails
ID Serval
serval:BIB_A97DE46A8D0C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Altered hepatic transport of immunoglobulin A in mice lacking the J chain
Périodique
Journal of Experimental Medicine
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
12/1995
Volume
182
Numéro
6
Pages
1905-11
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec 1
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec 1
Résumé
We have created J chain knockout mice to define the physiologic role of the J chain in immunoglobulin synthesis and transport. The J chain is covalently associated with pentameric immunoglobulin (Ig) M and dimeric IgA and is also expressed in most IgG-secreting cells. J chain-deficient mice have normal serum IgM and IgG levels but markedly elevated serum IgA. Although polymeric IgA was present in the mutant mice, a larger proportion of their serum IgA was monomeric than was found in wild-type mouse serum. Bile and fecal IgA levels were decreased in J chain-deficient mice compared with wild-type mice, suggesting inefficient transport of J chain-deficient IgA by hepatic polymeric immunoglobulin receptors (pIgR). The pIgR-mediated transport of serum-derived IgA from wild-type and mutant mice was assessed in Madin-Darby canine kidney (MDCK) cells transfected with the pIgR. These studies revealed selective transport by pIgR-expressing MDCK cells of wild-type IgA but not J chain-deficient IgA. We conclude that although the J chain is not required for IgA dimerization, it does affect the efficiency of polymerization or have a role in maintaining IgA dimer stability. Furthermore, the J chain is essential for efficient hepatic pIgR transport of IgA.
Mots-clé
Animals
Base Sequence
Bile/metabolism
Biological Transport
Cells, Cultured
DNA Primers/chemistry
Dogs
Genes, Immunoglobulin
Immunoglobulin A/chemistry/*metabolism
Immunoglobulin J-Chains/*metabolism
Liver/*metabolism
Macromolecular Substances
Mice
Mice, Knockout
Molecular Sequence Data
Restriction Mapping
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:53
Dernière modification de la notice
20/08/2019 16:13