The long-term culture of porcine urothelial cells and induction of urothelial stratification.

Détails

ID Serval
serval:BIB_A979B0546D90
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The long-term culture of porcine urothelial cells and induction of urothelial stratification.
Périodique
BJU International
Auteur⸱e⸱s
Ludwikowski B., Zhang Y.Y., Frey P.
ISSN
1464-4096
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
84
Numéro
4
Pages
507-514
Langue
anglais
Résumé
OBJECTIVE: To assess porcine urothelial cell cultures and the in vitro induction of urothelial stratification in long-term cultures, to study their morphological, functional and genetic behaviour, and thus provide potential autologous urothelium for tissue-engineered substitutes for demucosalized gastric or colonic tissue. MATERIALS AND METHODS: Primary cultures of porcine urothelium were established and the cells passaged thereafter. Cell specificity was confirmed by cytokeratin analysis, cell membrane stability assessed using lactate dehydrogenase leakage, cell de-differentiation by gamma-glutamyl transferase activity and genomic stability by karyotype investigations. Histology and scanning electron microscopy were performed to study the cultured cells and the stratified constructs. Furthermore, collagen matrices were tested as cell scaffolds. RESULTS: The cells were cultured for 180 days; 10 subcultures were established during this period. Stratification was induced in a culture flask and on a collagen matrix. Cytokeratins 7, 8, 17 and 18 were expressed in all cultures, and cell membranes were stable, with no evident de-differentiation. The cultures were stable in their genotype and no chromosomal aberrations were found. The histology and immunohistochemistry of the stratified porcine constructs, and cell membrane stability and cell de-differentiation, were compared with those in the human system. CONCLUSION: Pig and human urothelial cells can be cultured over a long period with no signs of senescence. Urothelial stratification can be induced in vitro. The collagen matrix seems to be an excellent scaffold, allowing cell adherence and growth.
Mots-clé
Animals, Cell Division, Cell Survival, Cells, Cultured, Collagen, Epithelial Cells, Humans, Immunohistochemistry, Karyotyping, Microscopy, Electron, Swine, Urothelium
Pubmed
Web of science
Création de la notice
28/02/2008 10:25
Dernière modification de la notice
20/08/2019 15:13
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