Parachlamydia acanthamoebae enters and multiplies within human macrophages and induces their apoptosis [corrected]

Détails

ID Serval
serval:BIB_A979408E8B74
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Parachlamydia acanthamoebae enters and multiplies within human macrophages and induces their apoptosis [corrected]
Périodique
Infection and Immunity
Auteur(s)
Greub  G., Mege  J. L., Raoult  D.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
10/2003
Volume
71
Numéro
10
Pages
5979-85
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Parachlamydia acanthamoebae is an obligately intracellular bacterium that naturally infects free-living amoebae. It is a potential human pathogen and may survive in human macrophages. We studied P. acanthamoebae entry into, and multiplication within, human monocyte-derived macrophages. After 8 h of incubation, 80% of macrophages were infected with a mean of 3.8 P. acanthamoebae organisms per cell. Electron microscopy demonstrated that parachlamydiae were in an intracellular vacuole. After infection with living organisms, the number of parachlamydiae per macrophage increased 4 times from day 0 to day 4, whereas heat-inactivated parachlamydiae were eliminated during the same period. Quantitative PCR confirmed that P. acanthamoebae replicates within macrophages. Transcriptional activity of P. acanthamoeba was detected by reverse transcription-PCR targeting the gene encoding ADP-ATP translocase (tlc). P. acanthamoebae exerted a cytopathic effect on macrophages. When macrophages were infected with living bacteria, their number decreased significantly from day 0 to day 4 due to apoptosis, as shown by annexin-V binding and electron microscopy. This study shows that P. acanthamoebae enters and multiplies within human macrophages before inducing their apoptosis.
Mots-clé
Apoptosis Bacterial Proteins/genetics Base Sequence Chlamydiales/genetics/growth & development/*pathogenicity/ultrastructure DNA, Bacterial/genetics Genes, Bacterial Humans Macrophages/*microbiology/ultrastructure Microscopy, Electron Mitochondrial ADP, ATP Translocases/genetics Reverse Transcriptase Polymerase Chain Reaction Vacuoles/microbiology/ultrastructure
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:28
Dernière modification de la notice
20/08/2019 16:13
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