Identification of Genes Affecting Vacuole Membrane Fragmentation in Saccharomyces cerevisiae.

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Ressource 1Télécharger: BIB_A959689C4C18.P001.pdf (1438.54 [Ko])
Etat: Public
Version: de l'auteur⸱e
ID Serval
serval:BIB_A959689C4C18
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of Genes Affecting Vacuole Membrane Fragmentation in Saccharomyces cerevisiae.
Périodique
PLoS One
Auteur⸱e⸱s
Michaillat L., Mayer A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2013
Volume
8
Numéro
2
Pages
e54160
Langue
anglais
Résumé
The equilibrium of membrane fusion and fission influences the volume and copy number of organelles. Fusion of yeast vacuoles has been well characterized but their fission and the mechanisms determining vacuole size and abundance remain poorly understood. We therefore attempted to systematically characterize factors necessary for vacuole fission. Here, we present results of an in vivo screening for deficiencies in vacuolar fragmentation activity of an ordered collection deletion mutants, representing 4881 non-essential genes of the yeast Saccharomyces cerevisiae. The screen identified 133 mutants with strong defects in vacuole fragmentation. These comprise numerous known fragmentation factors, such as the Fab1p complex, Tor1p, Sit4p and the V-ATPase, thus validating the approach. The screen identified many novel factors promoting vacuole fragmentation. Among those are 22 open reading frames of unknown function and three conspicuous clusters of proteins with known function. The clusters concern the ESCRT machinery, adaptins, and lipases, which influence the production of diacylglycerol and phosphatidic acid. A common feature of these factors of known function is their capacity to change membrane curvature, suggesting that they might promote vacuole fragmentation via this property.
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/03/2013 9:02
Dernière modification de la notice
20/08/2019 16:13
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