Airway epithelial IL-15 transforms monocytes into dendritic cells.

Détails

ID Serval
serval:BIB_A9397C8F9E9B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Airway epithelial IL-15 transforms monocytes into dendritic cells.
Périodique
American Journal of Respiratory Cell and Molecular Biology
Auteur(s)
Regamey N., Obregon C., Ferrari-Lacraz S., van Leer C., Chanson M., Nicod L.P., Geiser T.
ISSN
1044-1549
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
37
Numéro
1
Pages
75-84
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
IL-15 has recently been shown to induce the differentiation of functional dendritic cells (DCs) from human peripheral blood monocytes. Since DCs lay in close proximity to epithelial cells in the airway mucosa, we investigated whether airway epithelial cells release IL-15 in response to inflammatory stimuli and thereby induce differentiation and maturation of DCs. Alveolar (A549) and bronchial (BEAS-2B) epithelial cells produced IL-15 spontaneously and in a time- and dose-dependent manner after stimulation with IL-1beta, IFN-gamma, or TNF-alpha. Airway epithelial cell supernatants induced an increase of IL-15Ralpha gene expression in ex vivo monocytes, and stimulated DCs enhanced their IL-15Ralpha gene expression up to 300-fold. Airway epithelial cell-conditioned media induced the differentiation of ex vivo monocytes into partially mature DCs (HLA-DR+, DC-SIGN+, CD14+, CD80-, CD83+, CD86+, CCR3+, CCR6(+), CCR7-). Based on their phenotypic (CD123+, BDCA2+, BDCA4+, BDCA1(-), CD1a-) and functional properties (limited maturation upon stimulation with LPS and limited capacity to induce T cell proliferation), these DCs resembled plasmacytoid DCs. The effects of airway epithelial cell supernatants were largely blocked by a neutralizing monoclonal antibody to IL-15. Thus, our results demonstrate that airway epithelial cell-conditioned media have the capacity to differentiate monocytes into functional DCs, a process substantially mediated by epithelial-derived IL-15.
Mots-clé
Antibodies, Monoclonal/chemistry, Bronchi/metabolism, Cell Differentiation, Culture Media, Conditioned, Dendritic Cells/cytology, Dendritic Cells/drug effects, Epithelium/metabolism, Humans, Inflammation, Interferon-gamma/metabolism, Interleukin-15/administration & dosage, Interleukin-15/metabolism, Interleukin-15 Receptor alpha Subunit/metabolism, Lipopolysaccharides/metabolism, Monocytes/cytology, Monocytes/drug effects, Pulmonary Alveoli/metabolism, Tumor Necrosis Factor-alpha/metabolism
Pubmed
Web of science
Création de la notice
12/02/2010 16:43
Dernière modification de la notice
20/08/2019 16:13
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