Lack of functionally active Melan-A(26-35)-specific T cells in the blood of HLA-A2+ vitiligo patients.

Détails

ID Serval
serval:BIB_A9377307352D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Lack of functionally active Melan-A(26-35)-specific T cells in the blood of HLA-A2+ vitiligo patients.
Périodique
Journal of Investigative Dermatology
Auteur(s)
Adams S., Lowes M.A., O'Neill D.W., Schachterle S., Romero P., Bhardwaj N.
ISSN
1523-1747 (Electronic)
ISSN-L
0022-202X
Statut éditorial
Publié
Date de publication
2008
Volume
128
Numéro
8
Pages
1977-1980
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Vitiligo, a skin disorder characterized by the spontaneous destruction of melanocytes, is believed to be of autoimmune origin. We investigated the presence and functionality of CD8(+) T-cells specific for the melanocyte-associated antigens Melan-A, gp100, tyrosinase, and TRP-2 in the blood of HLA-A2(+) vitiligo patients. We enumerated antigen-specific CD8(+) T cells by major histocompatibility complex multimer staining directly ex vivo, as well as after 9 days of in vitro stimulation and assessed IFN-gamma secretion by enzyme-linked immunospot (Elispot) assay. Tyrosinase-, gp100-, or TRP-2-specific CD8(+) T cells could not be identified in the peripheral blood of individuals with vitiligo. Although Melan-A-specific T cells were detectable at levels comparable to Flu-MP-specific T cells by multimer staining, these lymphocytes did not express the skin-homing receptor cutaneous lymphocyte antigen, were phenotypically naïve (CD45RA(+)), and were unresponsive in the IFN-gamma Elispot assay, suggesting that they are unlikely to be involved in the etiopathogenesis of vitiligo.
Mots-clé
Antigens, Neoplasm/blood, Antigens, Neoplasm/metabolism, CD8-Positive T-Lymphocytes/metabolism, CD8-Positive T-Lymphocytes/pathology, Case-Control Studies, HLA-A2 Antigen/metabolism, Humans, Interferon-gamma/metabolism, MART-1 Antigen, Membrane Glycoproteins/blood, Membrane Glycoproteins/metabolism, Membrane Proteins/metabolism, Monophenol Monooxygenase/metabolism, Neoplasm Proteins/blood, Neoplasm Proteins/metabolism, Peptide Fragments/metabolism, Vitiligo/blood, Vitiligo/etiology, gp100 Melanoma Antigen
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/09/2009 18:36
Dernière modification de la notice
20/08/2019 16:13
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