Functional characterisation of serum DNase I in MRL-lpr/lpr mice

Détails

ID Serval
serval:BIB_A90612652FFF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Functional characterisation of serum DNase I in MRL-lpr/lpr mice
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Peitsch  M. C., Hesterkamp  T., Polzar  B., Mannherz  H. G., Tschopp  J.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
07/1992
Volume
186
Numéro
2
Pages
739-45
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 31
Résumé
The autosomal defect in Fas antigen leads CD4-CD8-T-cells to accumulate in lymph nodes and spleen of MRL-lpr/lpr mice. MRL-lpr/lpr mice present increased levels of DNase I as compared to the control strain MRL-+/+. This DNase I, which most probably originates from the accumulated CD4-CD8-T-cells, cleaves nuclear DNA with a strong preference for internucleosomal sites yielding, in the presence of both Ca2+ and Mg2+, a pattern of fragments typical for apoptosis. Furthermore, we show that this "apoptosis-ladder" can be obtained with purified DNase I in presence of normal serum.
Mots-clé
Animals Antigens, CD4/analysis Antigens, CD8/analysis Calcium/pharmacology Cell Death Cell Nucleus/*enzymology DNA/isolation & purification/metabolism Deoxyribonuclease I/*blood/isolation & purification/metabolism Kinetics Lymph Nodes/enzymology Magnesium/pharmacology Mice Mice, Mutant Strains Nuclear Proteins/isolation & purification Parotid Gland/enzymology Reference Values T-Lymphocyte Subsets/*enzymology
Pubmed
Web of science
Création de la notice
24/01/2008 16:19
Dernière modification de la notice
20/08/2019 16:13
Données d'usage