Nonselective versus selective inhibition of inducible nitric oxide synthase in experimental endotoxic shock
Détails
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Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Etat: Public
Version: Final published version
Licence: Non spécifiée
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
ID Serval
serval:BIB_A8CE9B40CA50
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nonselective versus selective inhibition of inducible nitric oxide synthase in experimental endotoxic shock
Périodique
Journal of Infectious Diseases
ISSN
0022-1899 (Print)
Statut éditorial
Publié
Date de publication
01/1998
Volume
177
Numéro
1
Pages
127-32
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
The effects of two nitric oxide synthase (NOS) inhibitors with different isoform selectivity were compared in a murine model of endotoxemia. Mice challenged with 70 mg/kg intraperitoneal (ip) lipopolysaccharide (LPS) were treated 6 h after LPS with either NG-gamma-L-arginine methyl ester (L-NAME, nonselective NOS inhibitor, 10-60 mg/kg), L-canavanine (selective inhibitor of inducible NOS, 50-300 mg/kg), or saline (0.2 mL) given ip. In a subset of mice, plasma concentrations of nitrate (NO breakdown product), lipase (pancreas injury), lactate dehydrogenase, and transaminases (liver injury) were measured 16 h after LPS. Although both inhibitors reduced plasma nitrate, they produced contrasting effects on survival and organ injury. L-NAME enhanced liver damage and tended to accelerate the time of death, while L-canavanine significantly reduced mortality and had no deleterious effects in terms of organ damage. These results indicate that nonselective NOS inhibitors are detrimental in endotoxic shock and support the potential usefulness of selective inducible NOS inhibitors in this setting.
Mots-clé
Animals
Canavanine/administration & dosage/adverse effects/*therapeutic use
Enzyme Inhibitors/administration & dosage/adverse effects/*therapeutic use
Female
L-Lactate Dehydrogenase/analysis/blood
Lipase/analysis/blood
Lipopolysaccharides/administration & dosage/pharmacology
Liver/pathology
Mice
NG-Nitroarginine Methyl Ester/administration & dosage/adverse
effects/*therapeutic use
Nitrates/analysis/blood
Nitric Oxide/metabolism
Nitric Oxide Synthase/*metabolism
Shock, Septic/*drug therapy/*metabolism
Transaminases/analysis/blood
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 10:37
Dernière modification de la notice
14/02/2022 8:56