High- and low-affinity binding sites for vasoactive intestinal peptide (VIP) in the rat kidney revealed by light microscopic autoradiography

Détails

ID Serval
serval:BIB_A880E260619C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
High- and low-affinity binding sites for vasoactive intestinal peptide (VIP) in the rat kidney revealed by light microscopic autoradiography
Périodique
Regulatory Peptides
Auteur(s)
Magistretti  P. J., Hof  P. R., Martin  J. L., Dietl  M., Palacios  J. M.
ISSN
0167-0115
Statut éditorial
Publié
Date de publication
11/1988
Peer-reviewed
Oui
Volume
23
Numéro
2
Pages
145-52
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
The distribution of VIP binding sites in rat kidney and adrenal gland has been examined by light microscopic autoradiography. A fully characterized mono-iodinated molecular form of VIP (M-125-I-VIP) which maintains the biological activity of the native peptide, was used for this study. Two types of VIP binding sites, with high and low affinity, have been identified. High affinity sites are associated with (i) glomerular structures in the cortex, (ii) the inner stripe of the outer medulla, possibly corresponding to Henle's loops and distal tubules, (iii) radiated structures in the inner zone of the medulla, likely to represent labeling of collecting ducts and/or vascular bundles and (iv) the adrenal cortex. Autoradiographic grains associated with low affinity sites are present diffusely throughout the renal cortex, possibly corresponding to labeling of tubular and/or vascular structures, and throughout the adrenal gland. These observations further delineate a role of VIP in renal and neuroendocrine function.
Mots-clé
Animals Autoradiography Iodine Radioisotopes/diagnostic use Kidney Glomerulus/*analysis Kidney Medulla/*analysis Male Rats Receptors, Gastrointestinal Hormone/*analysis Receptors, Vasoactive Intestinal Peptide Vasoactive Intestinal Peptide/*analysis
Pubmed
Web of science
Création de la notice
20/01/2008 19:22
Dernière modification de la notice
20/08/2019 16:12
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