Interleukin-1β inhibition prevents choroidal neovascularization and does not exacerbate photoreceptor degeneration.

Détails

ID Serval
serval:BIB_A8519F38F4E7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Interleukin-1β inhibition prevents choroidal neovascularization and does not exacerbate photoreceptor degeneration.
Périodique
American Journal of Pathology
Auteur⸱e⸱s
Lavalette S., Raoul W., Houssier M., Camelo S., Levy O., Calippe B., Jonet L., Behar-Cohen F., Chemtob S., Guillonneau X., Combadière C., Sennlaub F.
ISSN
1525-2191 (Electronic)
ISSN-L
0002-9440
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
178
Numéro
5
Pages
2416-2423
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The pro-inflammatory cytokine IL-1β has been shown to promote angiogenesis. It can have a neurotoxic or neuroprotective effect. Here, we have studied the expression of IL-1β in vivo and the effect of the IL-1 receptor antagonist on choroidal neovascularization (CNV) and retinal degeneration (RD). IL-1β expression significantly increased after laser injury (real time PCR) in C57BL/6 mice, in the C57BL/6 Cx3cr1(-/-) model of age-related macular degeneration (enzyme-linked immunoabsorbent assay), and in albino Wistar rats and albino BALB Cx3cr1(+/+) and Cx3cr1(-/-) mice (enzyme-linked immunoabsorbent assay) after light injury. IL-1β was localized to Ly6G-positive, Iba1-negative infiltrating neutrophils in laser-induced CNV as determined by IHC. IL-1 receptor antagonist treatment significantly inhibited CNV but did not affect Iba1-positive macrophage recruitment to the injury site. IL-1β significantly increased endothelial cell outgrowth in aortic ring assay independently of vascular endothelial growth factor, suggesting a direct effect of IL-1β on choroidal endothelial cell proliferation. Inhibition of IL-1β in light- and laser-induced RD models did not alter photoreceptor degeneration in Wistar rats, C57BL/6 mice, or RD-prone Cx3cr1(-/-) mice. Our results suggest that IL-1β inhibition might represent a valuable and safe alternative to inhibition of vascular endothelial growth factor in the control of CNV in the context of concomitant photoreceptor degeneration as observed in age-related macular degeneration.
Mots-clé
Animals, Choroidal Neovascularization/metabolism, Choroidal Neovascularization/prevention & control, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Interleukin-1beta/antagonists & inhibitors, Macrophages/metabolism, Mice, Mice, Inbred C57BL, Nerve Degeneration/metabolism, Nerve Degeneration/pathology, Nerve Regeneration/physiology, Photoreceptor Cells/metabolism, Photoreceptor Cells/pathology, Rats, Rats, Wistar, Retinal Degeneration/metabolism, Retinal Degeneration/prevention & control, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
22/08/2013 14:17
Dernière modification de la notice
20/08/2019 15:12
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