The vasodilatory effect of juxta-arteriolar microinjection of endothelinA receptor inhibitor in healthy and acute branch retinal vein occlusion minipig retinas.

Détails

ID Serval
serval:BIB_A847A58BF8EB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The vasodilatory effect of juxta-arteriolar microinjection of endothelinA receptor inhibitor in healthy and acute branch retinal vein occlusion minipig retinas.
Périodique
Investigative Ophthalmology and Visual Science
Auteur(s)
Stangos A.N., Petropoulos I.K., Pournaras J.A., Mendrinos E., Pournaras C.J.
ISSN
1552-5783 (Electronic)
ISSN-L
0146-0404
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
51
Numéro
4
Pages
2185-2190
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Purpose. To investigate the effect of the endothelin(A) receptor inhibitor BQ-123 on the retinal arteriolar vasculature in minipig retinas in normal eyes and eyes with acute branch retinal vein occlusion (BRVO). Methods. Seven healthy eyes of seven minipigs and six eyes of six minipigs with experimental BRVO were evaluated under systemic anesthesia. An intravitreal juxta-arteriolar microinjection of 30 microL BQ-123 0.61 microg/mL (pH 7.4) was performed in all but one eye from each group, into which the physiologic saline vehicle alone was injected. Vessel-diameter changes were measured with a retinal vessel analyzer. Results. In healthy minipig retinas (n = 6), arteriolar diameter (+/-SD) increased 6.19% +/- 3.55% (P < 0.05), 25.98% +/- 2.37% (P < 0.001), 23.65% +/- 1.2% (P < 0.001), and 16.84% +/- 1.95% (P < 0.001), at 1, 5, 10, and 15 minutes, respectively, after BQ-123 microinjection. Two hours after experimental BRVO (n = 5), the retinal arteriolar diameter had decreased (13.07% +/- 5.7%; P < 0.01). One, 5, 10, and 15 minutes after BQ-123 microinjection, retinal arteriolar diameter had increased by 7.14% +/- 3.3% (P < 0.01), 26.74% +/- 7.63% (P < 0.001), 23.67% +/- 6.4% (P < 0.001), and 16.09% +/- 3.41% (P < 0.001), respectively. Vehicle only injection had no vasoactive effect on physiologic or BRVO retinas. Conclusions. A significant increase in retinal arteriolar diameter was demonstrated after juxta-arteriolar BQ-123 microinjection in healthy and in acute BRVO minipig retinas. The results suggest a role for endothelin-1 in maintaining retinal basal arteriolar tone. Reversing the BRVO-related vasoconstriction by juxta-arteriolar BQ-123 microinjection could bring a new perspective to the management of BRVO.
Mots-clé
Acute Disease, Animals, Arterioles/physiology, Disease Models, Animal, Endothelin-1/physiology, Microinjections, Muscle, Smooth, Vascular/physiology, Peptides, Cyclic/administration & dosage, Receptor, Endothelin A/antagonists & inhibitors, Retinal Artery/physiology, Retinal Vein Occlusion/physiopathology, Swine, Swine, Miniature, Vasodilation/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/02/2012 15:20
Dernière modification de la notice
20/08/2019 15:12
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