Using flow cytometry for in situ monitoring of antimicrobial compound production in the biocontrol bacterium Pseudomonas fluorescens CHA0

Détails

ID Serval
serval:BIB_A840FFEBF409
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Collection
Publications
Institution
Titre
Using flow cytometry for in situ monitoring of antimicrobial compound production in the biocontrol bacterium Pseudomonas fluorescens CHA0
Titre de la conférence
IOBC/WPRS Bulletin
Auteur(s)
de Werra P., Huser A., Baehler E., Keel C., Maurhofer M.
ISBN
92-9067-185-6
ISSN-L
1027-3115
Statut éditorial
Publié
Date de publication
2006
Editeur scientifique
Raaijmakers J.M., Sikora R.A.
Volume
29
Série
IOBC/WPRS Bulletin
Pages
117-121
Langue
anglais
Notes
Working Group ,,Multitrophic Interactions in Soil". Proceedings of a Meeting at Wageningen (The Netherlands), 5 - 8 June, 2005.
Résumé
Pseudomonas fluorescens strain CHA0 is able to protect plants against a variety of pathogens, notably by producing the two antimicrobial compounds 2,4-diacetylphloroglucinol (DAPG) and pyoluteorin (PLT). The regulation of the expression of these compounds is affected by many biotic factors, such as fungal pathogens, rhizosphere bacteria as well as plant species. Therefore, the influence of some plant phenolic compounds on the expression of DAPG and PLT biosynthetic genes has been tested using GFP-based reporter, monitored by standard fluometry and flow cytometry. In situ experiments were also performed with cucumber plants. We found that several plant metabolites such as IAA and umbelliferone are able to modify significantly the expression of DAPG and PLT. The use of flow cytometry with autofluorescents proteins seems to be a promising method to study rhizobacteria-plant interactions.
Création de la notice
24/01/2008 14:51
Dernière modification de la notice
20/08/2019 16:12
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