Detection of new cross-reacting carcinoembryonic antigen(s) on cultured tumor cells by mixed hemadsorption assay.

Détails

ID Serval
serval:BIB_A83B4FB683D3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Detection of new cross-reacting carcinoembryonic antigen(s) on cultured tumor cells by mixed hemadsorption assay.
Périodique
Journal of the National Cancer Institute
Auteur⸱e⸱s
Dent P.B., Carrel S., Mach J.P.
ISSN
0027-8874 (Print)
ISSN-L
0027-8874
Statut éditorial
Publié
Date de publication
1980
Volume
64
Numéro
2
Pages
309-316
Langue
anglais
Résumé
Antisera highly specific for carcinoembryonic antigen (CEA) from New Zealand White rabbits and a goat reacted strongly in antibody binding tests with cultured tumor cell lines, irrespective of the ability of the cell lines to produce CEA. The most reactive were colon carcinoma and melanoma cell lines, the former known to produce CEA and the latter not associated with CEA production. The reactivity was not diminished by absorption with perchloric acid extracts of normal lung or spleen, whereas absoprtion with purified CEA preparations abolished the reactivity. Quantitative absorption studies indicated that reactivity against CEA-producing cell lines could be totally removed by absorption with other CEA-producing lines but not with melanoma cell lines. Reactivity against melanoma cell lines could be completely removed by colon carcinoma cells as well as by melanoma cells. Antisera raised against purified CEA, after absorption with extracts of normal lung, still contained two populations of antibodies, one that binds a newly described antigen cross-reacting with CEA which is present on melanoma cells.
Mots-clé
Animals, Antibodies, Neoplasm, Antibody Specificity, Carcinoembryonic Antigen/immunology, Cell Line, Colonic Neoplasms/immunology, Cross Reactions, Epitopes, Female, Goats, Humans, Melanoma/immunology, Rabbits, Uterine Cervical Neoplasms/immunology
Pubmed
Web of science
Création de la notice
25/04/2013 10:35
Dernière modification de la notice
20/08/2019 15:12
Données d'usage