Plasminogen activation in synovial tissues: differences between normal, osteoarthritis, and rheumatoid arthritis joints

Détails

ID Serval
serval:BIB_A81FB9C3B000
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Plasminogen activation in synovial tissues: differences between normal, osteoarthritis, and rheumatoid arthritis joints
Périodique
Annals of the Rheumatic Diseases
Auteur⸱e⸱s
Busso  N., Peclat  V., So  A., Sappino  A. P.
ISSN
0003-4967 (Print)
Statut éditorial
Publié
Date de publication
09/1997
Volume
56
Numéro
9
Pages
550-7
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
OBJECTIVE: To analyse the functional activity of the plasminogen activators urokinase (uPA) and tissue type plasminogen activator (tPA) in human synovial membrane, and to compare the pattern of expression between normal, osteoarthritic, and rheumatoid synovium. The molecular mechanisms underlying differences in PA activities between normal and pathological synovial tissues have been further examined. METHODS: Synovial membranes from seven normal (N) subjects, 14 osteoarthritis (OA), and 10 rheumatoid arthritis (RA) patients were analysed for plasminogen activator activity by conventional zymography and in situ zymography on tissue sections. The tissue distribution of uPA, tPA, uPA receptor (uPAR), and plasminogen activator inhibitor type-1 (PAI-1) was studied by immunohistochemistry. uPA, tPA, uPAR, and PAI-1 mRNA values and mRNA distribution were assessed by northern blot and in situ hybridisations respectively. RESULTS: All normal and most OA synovial tissues expressed predominantly tPA catalysed proteolytic activity mainly associated to the synovial vasculature. In some OA, tPA activity was expressed together with variable amounts of uPA mediated activity. By contrast, most RA synovial tissues exhibited considerably increased uPA activity over the proliferative lining areas, while tPA activity was reduced when compared with N and OA synovial tissues. This increase in uPA activity was associated with increased levels of uPA antigen and its corresponding mRNA, which were localised over the synovial proliferative lining areas. In addition, in RA tissues, expression of the specific uPA receptor (uPAR) and of the plasminogen activator inhibitor-type 1
Mots-clé
Arthritis, Rheumatoid/*enzymology Blotting, Northern Humans Immunohistochemistry In Situ Hybridization Osteoarthritis/*enzymology Plasminogen Activator Inhibitor 1/analysis/genetics Plasminogen Activators/*analysis/genetics RNA, Messenger/analysis Receptors, Cell Surface/analysis/genetics Synovial Membrane/*enzymology Tissue Plasminogen Activator/analysis/genetics Urinary Plasminogen Activator/analysis/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 8:38
Dernière modification de la notice
20/08/2019 15:12
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