Genomic Characterization of an Extensively Drug-Resistant Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Clinical Isolate Co-Producing Two Carbapenemases and a 16S rRNA Methylase.
Détails
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_A7BED5ECE8DD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genomic Characterization of an Extensively Drug-Resistant Extra-Intestinal Pathogenic (ExPEC) Escherichia coli Clinical Isolate Co-Producing Two Carbapenemases and a 16S rRNA Methylase.
Périodique
Antibiotics
ISSN
2079-6382 (Print)
ISSN-L
2079-6382
Statut éditorial
Publié
Date de publication
26/10/2022
Peer-reviewed
Oui
Volume
11
Numéro
11
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Publication Status: epublish
Résumé
An extensively drug-resistant Escherichia coli clinical isolate (N1606) belonging to Sequence Type 361 was recovered from the urine of a patient hospitalized in Switzerland. The strain showed resistance to virtually all β-lactams including the latest generation antibiotics cefiderocol and aztreonam-avibactam. Whole genome sequencing revealed that it possessed two carbapenemase-encoding genes, namely bla <sub>NDM-5</sub> and bla <sub>KPC-3</sub> , and a series of additional β-lactamase genes, including bla <sub>CTX-M-15</sub> and bla <sub>SHV-11</sub> encoding extended-spectrum β-lactamases (ESBLs), bla <sub>CMY-145</sub> encoding an AmpC-type cephalosporinase, and bla <sub>OXA-1</sub> encoding a narrow-spectrum class D ß-lactamase. Most of these resistance genes were located on plasmids (IncFII-FIA, IncX3, IncIγ, IncFII). That strain exhibited also a four amino-acid insertion in its penicillin-binding protein 3 (PBP3) sequence, namely corresponding to YRIN. Complete genome analysis revealed that this E. coli isolate carried virulence factors (sitA, gad, hra, terC, traT, and cia) and many other non-β-lactam resistance determinants including rmtB, tet(A), dfrA17 (two copies), aadA1, aadA5 (two copies), sul1 (two copies), qacE (two copies), qepA, mdf(A), catA1, erm(B), mph(A), and qnrS1, being susceptible only to tigecycline, colistin and fosfomycin. In conclusion, we described here the phenotypic and genome characteristics of an extensively drug-resistant (XDR) E. coli ST361 being recognized as an emerging clone worldwide.
Mots-clé
Escherichia coli, KPC, NDM, carbapenemase, plasmid
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/02/2023 15:25
Dernière modification de la notice
11/11/2023 8:19