Spectral-domain optical coherence tomography of the rodent eye: highlighting layers of the outer retina using signal averaging and comparison with histology.

Détails

ID Serval
serval:BIB_A70543FF8FD7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Spectral-domain optical coherence tomography of the rodent eye: highlighting layers of the outer retina using signal averaging and comparison with histology.
Périodique
PloS one
Auteur(s)
Berger A., Cavallero S., Dominguez E., Barbe P., Simonutti M., Sahel J.A., Sennlaub F., Raoul W., Paques M., Bemelmans A.P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
9
Numéro
5
Pages
e96494
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
Spectral-Domain Optical Coherence Tomography (SD-OCT) is a widely used method to observe retinal layers and follow pathological events in human. Recently, this technique has been adapted for animal imaging. This non-invasive technology brings a cross-sectional visualization of the retina, which permits to observe precisely each layer. There is a clear expansion of the use of this imaging modality in rodents, thus, a precise characterization of the different outer retinal layers observed by SD-OCT is now necessary to make the most of this technology. The identification of the inner strata until the outer nuclear layer has already been clearly established, while the attribution of the layers observed by SD-OCT to the structures corresponding to photoreceptors segments and retinal pigment epithelium is much more questionable. To progress in the understanding of experimental SD-OCT imaging, we developed a method for averaging SD-OCT data to generate a mean image allowing to better delineate layers in the retina of pigmented and albino strains of mice and rats. It allowed us to locate precisely the interface between photoreceptors and retinal pigment epithelium and to identify unambiguously four layers corresponding to the inner and outer parts of photoreceptors segments. We show that the thickness of the various layers can be measured as accurately in vivo on SD-OCT images, than post-mortem by a morphometric analysis of histological sections. We applied SD-OCT to different models and demonstrated that it allows analysis of focal or diffuse retinal pathological processes such as mutation-dependent damages or light-driven modification of photoreceptors. Moreover, we report a new method of combined use of SD-OCT and integration to quantify laser-induced choroidal neovascularization. In conclusion, we clearly demonstrated that SD-OCT represents a valuable tool for imaging the rodent retina that is at least as accurate as histology, non-invasive and allows longitudinal follow-up of the same animal.
Mots-clé
Animals, Choroidal Neovascularization/diagnosis, Choroidal Neovascularization/etiology, Choroidal Neovascularization/pathology, Laser Coagulation/adverse effects, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Reproducibility of Results, Retina/anatomy & histology, Retinal Degeneration/diagnosis, Retinal Degeneration/genetics, Retinal Degeneration/pathology, Rhodopsin/deficiency, Rhodopsin/genetics, Sensitivity and Specificity, Tomography, Optical Coherence/methods
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/07/2020 11:10
Dernière modification de la notice
16/07/2020 9:43
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