Activated B cells express functional Fas ligand.

Détails

ID Serval
serval:BIB_A6BC9CA56CF8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Activated B cells express functional Fas ligand.
Périodique
European journal of immunology
Auteur⸱e⸱s
Hahne M., Renno T., Schroeter M., Irmler M., French L., Bornard T., MacDonald H.R., Tschopp J.
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
1996
Peer-reviewed
Oui
Volume
26
Numéro
3
Pages
721-724
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Fas ligand (FasL, Apo-1L) is a member of the tumor necrosis factor protein family and binding to its receptor (Fas, Apo-1, CD95) triggers cell death through apoptosis. Ligand expression is restricted to cells with known cytolytic activity and found on hematopoietic cells of the T cell and natural killer lineage. Here we provide evidence that B lymphocytes can express FasL. Flow cytometric analysis revealed that FasL is expressed on the surface of B cells upon stimulation with either lipopolysaccharide or phorbol 12-myristate 13-acetate/ionomycin. FasL expression on activated B cells was confirmed by western blot and reverse transcriptase polymerase chain reaction analysis. FasL on B cells is functional since lipopolysaccharide-activated B lymphocytes derived from wild type, but not from gld mutant mice, were able to kill Fas-sensitive target cells. Our data suggest that the Fas system may contribute to the control of B cell homeostasis.
Mots-clé
Amino Acid Sequence, Animals, Antigens, CD95/biosynthesis, Antigens, CD95/physiology, B-Lymphocytes/drug effects, B-Lymphocytes/immunology, Base Sequence, Cytotoxicity, Immunologic/drug effects, Fas Ligand Protein, Ligands, Lipopolysaccharides/pharmacology, Lymphocyte Activation, Membrane Glycoproteins/biosynthesis, Membrane Glycoproteins/physiology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Tumor Necrosis Factor-alpha/physiology
Pubmed
Web of science
Création de la notice
24/01/2008 15:18
Dernière modification de la notice
20/08/2019 15:11
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