Generation of a tightly regulated all-cis beta cell-specific tetracycline-inducible vector.

Détails

ID Serval
serval:BIB_A6B900EAA5C2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Generation of a tightly regulated all-cis beta cell-specific tetracycline-inducible vector.
Périodique
Biotechniques
Auteur(s)
Bulat N., Widmann C.
ISSN
0736-6205[print], 0736-6205[linking]
Statut éditorial
Publié
Date de publication
2008
Volume
45
Numéro
4
Pages
411, 414, 416 passim
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Ability to induce protein expression at will in a cell is a powerful strategy used by scientists to better understand the function of a protein of interest. Various inducible systems have been designed in eukaryotic cells to achieve this goal. Most of them rely on two distinct vectors, one encoding a protein that can regulate transcription by binding a compound X, and one hosting the cDNA encoding the protein of interest placed downstream of promoter sequences that can bind the protein regulated by compound X (e.g., tetracycline, ecdysone). The commercially available systems are not designed to allow cell- or tissue-specific regulated expression. Additionally, although these systems can be used to generate stable clones that can be induced to express a given protein, extensive screening is often required to eliminate the clones that display poor induction or high basal levels. In the present report, we aimed to design a pancreatic beta cell-specific tetracycline-inducible system. Since the classical two-vector based tetracycline-inducible system proved to be unsatisfactory in our hands, a single vector was eventually designed that allowed tight beta cell-specific tetracycline induction in unselected cell populations.
Mots-clé
Animals, COS Cells, Cercopithecus aethiops, GTPase-Activating Proteins/chemistry, GTPase-Activating Proteins/metabolism, Gene Expression Regulation/drug effects, Genetic Techniques, Genetic Vectors/genetics, Humans, Insulin-Secreting Cells/drug effects, Insulin-Secreting Cells/metabolism, Mice, Organ Specificity/drug effects, Plasmids/genetics, Response Elements/genetics, Tetracycline/pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
29/01/2009 23:14
Dernière modification de la notice
20/08/2019 16:11
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