BDNF and DPP-IV in polyps and middle turbinates epithelial cells

Détails

ID Serval
serval:BIB_A685CD7325D6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
BDNF and DPP-IV in polyps and middle turbinates epithelial cells
Périodique
Rhinology
Auteur(s)
Jornot  L., Grouzmann  E., Lacroix  J. S., Rochat  T.
ISSN
0300-0729 (Print)
Statut éditorial
Publié
Date de publication
06/2007
Volume
45
Numéro
2
Pages
129-33
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
HYPOTHESIS: Neuropeptides released from sensory nerves may contribute to airway inflammation, particularly if their metabolism is impaired through defective inactivation and/or increased production. In the airways, neuropeptides are subjected to degradation by enzymes such as dipeptidyl peptidase (DPP-IV), and are upregulated by neurotrophins such as brain derived neurotrophic factor (BDNF). We therefore assessed in primary human nasal epithelial cells the expression of DPP-IV and BDNF under basal and inflammatory conditions. METHODS: Human epithelial cells were isolated from nasal polyps and middle turbinates, and grown on collagen-coated polycarbonate filters with an air liquid-interface. After three weeks of culture, constitutive cellular expression of DPP-IV and BDNF was assessed by measuring its activity and by ELISA, respectively. To mimick in vivo inflammatory conditions, cells were exposed apically and basolaterally to 50 ng/ml of TNFalpha, IL-1beta, and IFN-gamma for two days. DPP-IV activity and BDNF protein expression were measured in cell lysates and in the apical and basolateral media. RESULTS: Constitutive DPP-IV activity was similar in polyps and turbinates cells. In contrast, polyps epithelial cells expressed higher amounts of BDNF compared to turbinates derived cells. On the other hand, TNFalpha, IL-1beta, and IFN-gamma did not affect DPP-IV activity but significantly increased the cellular expression and the basolateral secretion of BDNF. CONCLUSIONS: Our data show for the first time that primary human airway epithelial cells produced DPP-IV and BDNF under basal conditions. Furthermore, the production and secretion of BDNF were markedly increased in response to pro-inflammatory cytokines, confirming the involvement of BDNF in airway inflammation.
Pubmed
Web of science
Création de la notice
25/01/2008 11:55
Dernière modification de la notice
20/08/2019 16:11
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