3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy.

Détails

Ressource 1Télécharger: BIB_A662BC1558A2.P001.pdf (4124.47 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_A662BC1558A2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy.
Périodique
Nucleic Acids Research
Auteur(s)
Amzallag A., Vaillant C., Jacob M., Unser M., Bednar J., Kahn J.D., Dubochet J., Stasiak A., Maddocks J.H.
ISSN
1362-4962[electronic], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
34
Numéro
18
Pages
e125
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
We use cryo-electron microscopy to compare 3D shapes of 158 bp long DNA minicircles that differ only in the sequence within an 18 bp block containing either a TATA box or a catabolite activator protein binding site. We present a sorting algorithm that correlates the reconstructed shapes and groups them into distinct categories. We conclude that the presence of the TATA box sequence, which is believed to be easily bent, does not significantly affect the observed shapes.
Mots-clé
Algorithms, Base Sequence, Binding Sites, Cluster Analysis, Cryoelectron Microscopy, Cyclic AMP Receptor Protein/metabolism, DNA, Circular/chemistry, DNA, Circular/metabolism, Imaging, Three-Dimensional, Molecular Sequence Data, Motion, Nucleic Acid Conformation, Software, TATA Box
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
20/08/2019 16:11
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