Highly sensitive microassay for aldosterone in unextracted plasma: comparison with two other methods

Détails

ID Serval
serval:BIB_A57F88A3781E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Highly sensitive microassay for aldosterone in unextracted plasma: comparison with two other methods
Périodique
Journal of Laboratory and Clinical Medicine
Auteur(s)
Nussberger  J., Waeber  B., Brunner  H. R., Burris  J. F., Vetter  W.
ISSN
0022-2143 (Print)
Statut éditorial
Publié
Date de publication
11/1984
Volume
104
Numéro
5
Pages
789-96
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
A direct radioimmunoassay for aldosterone in unextracted plasma was developed using a highly sensitive and specific antiserum and 125I-labeled aldosterone. This new method was compared with the previously used assay based on methylene chloride extraction and column chromatography and with a micromethod utilizing methylene chloride extraction alone. Concentrations of 2 pg/ml aldosterone can be measured in 25 microliter plasma. The within-assay coefficient of variation ranged between 4.0% and 5.3% over a sixfold range of aldosterone concentrations, and the between-assay coefficient of variation ranged between 5.0% and 9.4% over a tenfold range. The correlation coefficients among the three methods varied between 0.97 and 0.99, and results were virtually identical between the new direct and the standard chromatography methods. Normal values on unrestricted salt intake were 68 +/- 28 pg/ml (n = 30, mean +/- SD) in seated humans. In conclusion, this is an extremely simple, truly direct microradioimmunoassay for plasma aldosterone that is accurate over a wide range of plasma concentrations, is exquisitely sensitive, and provides results identical to those obtained with established methods.
Mots-clé
Aldosterone/*blood Chromatography, Gel Cross Reactions Evaluation Studies Humans Methylene Chloride Microchemistry/methods Radioimmunoassay/methods Reference Values
Pubmed
Web of science
Création de la notice
05/03/2008 17:39
Dernière modification de la notice
20/08/2019 16:10
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