Fibrin matrix for suspension of regenerative cells in an artificial nerve conduit.

Détails

ID Serval
serval:BIB_A579291CA764
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Fibrin matrix for suspension of regenerative cells in an artificial nerve conduit.
Périodique
Journal of Plastic, Reconstructive & Aesthetic Surgery
Auteur(s)
Kalbermatten D.F., Kingham P.J., Mahay D., Mantovani C., Pettersson J., Raffoul W., Balcin H., Pierer G., Terenghi G.
ISSN
1748-6815
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
61
Numéro
6
Pages
669-675
Langue
anglais
Résumé
Peripheral nerve injury presents with specific problems of neuronal reconstructions, and from a clinical viewpoint a tissue engineering approach would facilitate the process of repair and regeneration. We have previously used artificial nerve conduits made from bioresorbable poly-3-hydroxybutyrate (PHB) in order to refine the ways in which peripheral nerves are repaired and reconnected to the target muscles and skin. The addition of Schwann cells (SC) or differentiated mesenchymal stem cells (dMSC) to the conduits enhances regeneration. In this study, we have used a matrix based on fibrin (Tisseel) to fill optimally the nerve-conduits with cells. In vitro analysis showed that both SC and MSC adhered significantly better to PHB in the presence of fibrin and cells continued to maintain their differentiated state. Cells were more optimally distributed throughout the conduit when seeded in fibrin than by delivery in growth medium alone. Transplantation of the nerve conduits in vivo showed that cells in combination with fibrin matrix significantly increased nerve regeneration distance (using PGP9.5 and S100 distal and proximal immunohistochemistry) when compared with empty PHB conduits. This study shows the beneficial combinatory effect of an optimised matrix, cells and conduit material as a step towards bridging nerve gaps which should ultimately lead to improved functional recovery following nerve injury.
Mots-clé
Absorbable Implants, Animals, Cell Adhesion, Cell Differentiation, Fibrin Tissue Adhesive, Hydroxybutyrates, Mesenchymal Stem Cells, Nerve Regeneration, Peripheral Nerves, Polyesters, Rats, Rats, Sprague-Dawley, Schwann Cells, Tissue Engineering
Pubmed
Web of science
Création de la notice
18/02/2008 10:27
Dernière modification de la notice
20/08/2019 16:10
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